| pubmed-article:16530193 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16530193 | lifeskim:mentions | umls-concept:C0029235 | lld:lifeskim |
| pubmed-article:16530193 | lifeskim:mentions | umls-concept:C0010798 | lld:lifeskim |
| pubmed-article:16530193 | lifeskim:mentions | umls-concept:C0205182 | lld:lifeskim |
| pubmed-article:16530193 | lifeskim:mentions | umls-concept:C1704259 | lld:lifeskim |
| pubmed-article:16530193 | lifeskim:mentions | umls-concept:C1705987 | lld:lifeskim |
| pubmed-article:16530193 | lifeskim:mentions | umls-concept:C0320458 | lld:lifeskim |
| pubmed-article:16530193 | lifeskim:mentions | umls-concept:C1523987 | lld:lifeskim |
| pubmed-article:16530193 | lifeskim:mentions | umls-concept:C0599871 | lld:lifeskim |
| pubmed-article:16530193 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:16530193 | pubmed:dateCreated | 2006-3-28 | lld:pubmed |
| pubmed-article:16530193 | pubmed:abstractText | A cDNA encoding a C20 Delta8-desaturase was isolated from the free-living soil amoeba, Acanthamoeba castellanii and functionally characterised by heterologous expression. The open reading frame of the A. castellanii C20 Delta8-desaturase showed similarity to other microsomal front-end desaturases, but the N-terminal domain contained a variant form of the conserved heme-binding motif in which H-P-G-G is replaced by H-P-A-G. Co-expression of the A. castellani Delta8-desaturase with the Isochrysis galbana Delta9-elongase in transgenic Arabidopsis plants confirmed the activity observed in yeast and its role in the alternative pathway for C20 polyunsaturated fatty acid synthesis. Acyl-CoA profiles of these transgenic plants revealed an unexpected accumulation of C20 fatty acids in the acyl-CoA pool. This is the first report of an alternative pathway C20 Delta8-desaturase from a non-photosynthetic organism, and also the first report of a front-end desaturase lacking the canonical cytochrome b5 domain. | lld:pubmed |
| pubmed-article:16530193 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16530193 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16530193 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16530193 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16530193 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16530193 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16530193 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16530193 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16530193 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16530193 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:16530193 | pubmed:issn | 0014-5793 | lld:pubmed |
| pubmed-article:16530193 | pubmed:author | pubmed-author:NapierJohnath... | lld:pubmed |
| pubmed-article:16530193 | pubmed:author | pubmed-author:SayanovaOlgaO | lld:pubmed |
| pubmed-article:16530193 | pubmed:author | pubmed-author:QiBaoxiuB | lld:pubmed |
| pubmed-article:16530193 | pubmed:author | pubmed-author:LazarusColin... | lld:pubmed |
| pubmed-article:16530193 | pubmed:author | pubmed-author:HaslamRichard... | lld:pubmed |
| pubmed-article:16530193 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16530193 | pubmed:day | 3 | lld:pubmed |
| pubmed-article:16530193 | pubmed:volume | 580 | lld:pubmed |
| pubmed-article:16530193 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16530193 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16530193 | pubmed:pagination | 1946-52 | lld:pubmed |
| pubmed-article:16530193 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:16530193 | pubmed:meshHeading | pubmed-meshheading:16530193... | lld:pubmed |
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| pubmed-article:16530193 | pubmed:meshHeading | pubmed-meshheading:16530193... | lld:pubmed |
| pubmed-article:16530193 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16530193 | pubmed:articleTitle | The alternative pathway C20 Delta8-desaturase from the non-photosynthetic organism Acanthamoeba castellanii is an atypical cytochrome b5-fusion desaturase. | lld:pubmed |
| pubmed-article:16530193 | pubmed:affiliation | Rothamsted Research, Harpenden, Herts AL5 2JQ, UK. | lld:pubmed |
| pubmed-article:16530193 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16530193 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16530193 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16530193 | lld:pubmed |
