|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
8
|
|
pubmed:dateCreated |
2006-3-28
|
|
pubmed:abstractText |
The G-protein regulatory (GPR) motif is a approximately 25 amino acid sequence that stabilizes the GDP-bound conformation of Gialpha. To identify additional GPR motifs, we expanded a motif-based search strategy and identified an additional 4 mammalian proteins (WAVE1-3, rat GHRH) and 10 plant proteins with candidate GPR motifs. The WAVE1 GPR peptide inhibited GTPgammaS binding to purified G-protein. Endogenous Gialpha and WAVE1 coimmunoprecipitated from brain lysates. A WAVE1-G-protein complex was also observed following transfection of COS7 cells with Gialpha3 and WAVE1. The docking of Gialpha within a WAVE1 scaffolding complex may facilitate dynamic cycling and/or targeting for efficient and localized control of actin polymerization.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Apr
|
|
pubmed:issn |
0014-5793
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
3
|
|
pubmed:volume |
580
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1993-8
|
|
pubmed:dateRevised |
2009-11-19
|
|
pubmed:meshHeading |
pubmed-meshheading:16530190-Amino Acid Motifs,
pubmed-meshheading:16530190-Amino Acid Sequence,
pubmed-meshheading:16530190-Animals,
pubmed-meshheading:16530190-COS Cells,
pubmed-meshheading:16530190-Cercopithecus aethiops,
pubmed-meshheading:16530190-GTP-Binding Protein alpha Subunits, Gi-Go,
pubmed-meshheading:16530190-GTP-Binding Proteins,
pubmed-meshheading:16530190-Guanine Nucleotides,
pubmed-meshheading:16530190-Guanosine 5'-O-(3-Thiotriphosphate),
pubmed-meshheading:16530190-Molecular Sequence Data,
pubmed-meshheading:16530190-Protein Binding,
pubmed-meshheading:16530190-Rats,
pubmed-meshheading:16530190-Signal Transduction,
pubmed-meshheading:16530190-Wiskott-Aldrich Syndrome Protein Family
|
|
pubmed:year |
2006
|
|
pubmed:articleTitle |
Identification and characterization of a G-protein regulatory motif in WAVE1.
|
|
pubmed:affiliation |
Department of Pharmacology and Experimental Therapeutics, Louisiana State University Health Sciences Center, 1901 Perdido Street, New Orleans, LA 70112, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|
