Linked Life Data

1652996

Source:http://linkedlifedata.com/resource/pubmed/id/1652996

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1991-10-8
pubmed:abstractText
One hundred forty eight liver needle biopsies, comprising 88 consecutive biopsies from patients with clinically diagnosed or suspected alcoholic liver disease and 60 selected biopsies from non-alcoholics, were immunostained for the cell stress protein ubiquitin (Ub). Ub + cells were detected in all of 33 biopsies with alcoholic hepatitis (AH). Practically all Mallory bodies (MBs) showed intense Ub-staining. In addition, many cells revealed Ub + granules lying aggregated (pre-MBs) or dispersed in the cytoplasm of ballooned cells. The mean number of Ub + cells in 10 biopsies with AH was more than 30 times the number of MBs and more than 6 times the number of MBs + pre-MBs found in adjacent Haematoxylin-Eosin (HE) stained sections. Among 55 biopsies from alcoholic patients without AH (i.e. without MBs or pre-MBs in HE stained sections), Ub + cells occurred in eight (14.5%). Among the 60 selected biopsies from non-alcoholic patients with liver diseases in which hepatocyte ballooning and occasionally MBs are seen, a few Ub + cells were revealed in two out of ten with primary biliary cirrhosis (one of which also showed MBs) but none in biopsies with primary sclerosing cholangitis, long-standing large duct obstruction, or various hepatitides. Thus Ub-immunostaining appears to be a highly sensitive and specific method in the detection of MBs and MB precursor stages, making it a valuable tool in the study of alcoholic liver disease, and particularly a more objective method (compared to conventional staining methods) of diagnosing alcoholic hepatitis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0903-465X
pubmed:author
pubmed:issnType
Print
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
46-52
pubmed:dateRevised
2007-7-24
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Ubiquitin: an immunohistochemical marker of Mallory bodies and alcoholic liver disease.
pubmed:affiliation
Institute of Pathology, Aalborg Hospital, Denmark.
pubmed:publicationType
Journal Article