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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1991-10-2
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pubmed:abstractText |
We describe an acid phosphatase enzyme (EC 3.1.3.2) that is localized to the intestine of the nematode Caenorhabditis elegans and that should serve as a convenient biochemical marker for gut differentiation. In adult worms, acid phosphatase activity is located along the edge of the gut lumen in the vicinity of the intestinal brush border. All but the anterior six cells of the intestine stain for phosphatase activity; the nonstaining cells all descend from the Ea(l/r)(a/p)a cells. Acid phosphatase activity is low in oocytes and early embryos but increases substantially when embryos reach late morphogenesis stage; this increase corresponds to the appearance of a major band of acid phosphatase activity detectable on isoelectric focusing gels. We designate this band as the product of the pho-1 gene. The pattern of acid phosphatase expression in several embryonic mutants suggests that pho-1 expression in the developing intestine is lineage autonomous. We induced an isoelectric focusing variant in the pho-1 enzyme and used this to map the pho-1 locus about 1.5 map units to the left of center of chromosome II. We purified the pho-1 enzyme to homogeneity (6500-fold purification; 4% recovery of activity); the pho-1 acid phosphatase is a homodimeric glycoprotein with a subunit molecular weight of 55,000 Da. This paper establishes a new experimental system with which to investigate the molecular basis of lineage-specific gene expression during C. elegans development.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0012-1606
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
147
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
133-43
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1652526-Acid Phosphatase,
pubmed-meshheading:1652526-Animals,
pubmed-meshheading:1652526-Biological Markers,
pubmed-meshheading:1652526-Caenorhabditis,
pubmed-meshheading:1652526-Chromatography,
pubmed-meshheading:1652526-Chromatography, Affinity,
pubmed-meshheading:1652526-Chromatography, Gel,
pubmed-meshheading:1652526-Chromatography, Ion Exchange,
pubmed-meshheading:1652526-Durapatite,
pubmed-meshheading:1652526-Embryo, Nonmammalian,
pubmed-meshheading:1652526-Histocytochemistry,
pubmed-meshheading:1652526-Hydroxyapatites,
pubmed-meshheading:1652526-Intestines,
pubmed-meshheading:1652526-Isoelectric Focusing,
pubmed-meshheading:1652526-Kinetics
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pubmed:year |
1991
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pubmed:articleTitle |
An acid phosphatase as a biochemical marker for intestinal development in the nematode Caenorhabditis elegans.
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pubmed:affiliation |
Department of Medical Biochemistry, University of Calgary, Alberta, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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