Linked Life Data

1652349

Source:http://linkedlifedata.com/resource/pubmed/id/1652349

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1991-10-3
pubmed:abstractText
The purification of voltage-dependent sodium channels from mantle muscles of the squid is monitored through increase in the specific binding of tritiated saxitoxin (3H-STX) of membrane extracts solubilized with Lubrol-PX, chromatographed over an ion-exchange resin then through an affinity column coupling lectins (WGA) and Sepharose. The achieved purification factor is 300 and the electrophoretic pattern of purified fractions shows a single band at 260 kD. Reincorporation of these fractions into planar lipid bilayers yields two conductance levels (in 0.5 M NaCl). The most frequently observed level, at 15 pS, is in agreement with in situ recordings. The other level, at 150 pS, equally blocked by tetrodotoxin, could be explained by some flexibility of the open state or alternatively by cooperative interactions between channels.
pubmed:language
fre
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0764-4469
pubmed:author
pubmed:issnType
Print
pubmed:volume
312
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
277-84
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
[Purification of sodium channel from squid mantle and reincorporation in planar lipid bilayers].
pubmed:affiliation
Laboratoire Polymères, Biopolymères, Membranes, U.R.A.-C.N.R.S., n.500, Faculté des Sciences et Techniques, Mont-Saint-Aignan.
pubmed:publicationType
Journal Article, English Abstract