Source:http://linkedlifedata.com/resource/pubmed/id/16522180
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
7
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pubmed:dateCreated |
2006-3-8
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pubmed:abstractText |
Vasostatin has previously been expressed in fused form or in inclusion body form in Escherichia coli. Here the protein was expressed in soluble non-fusion form in BL21(DE3)pLysS by IPTG induction. The expression level of vasostatin was about 15% of the total cellular protein. The expressed vasostatin was purified and its biological activity was investigated by an endothelial cell proliferation assay.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0929-8665
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
659-61
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pubmed:meshHeading |
pubmed-meshheading:16522180-Calreticulin,
pubmed-meshheading:16522180-Cell Line,
pubmed-meshheading:16522180-Cell Proliferation,
pubmed-meshheading:16522180-Endothelial Cells,
pubmed-meshheading:16522180-Escherichia coli,
pubmed-meshheading:16522180-Gene Expression,
pubmed-meshheading:16522180-Peptide Fragments,
pubmed-meshheading:16522180-Recombinant Proteins,
pubmed-meshheading:16522180-Solubility
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pubmed:year |
2005
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pubmed:articleTitle |
Expression and purification of soluble non-fusion vasostatin in Escherichia coli.
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pubmed:affiliation |
Biopharmaceutical Research & Development Center, Pharmacy College, Jinan University, Guangzhou 510632, P. R. China.
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pubmed:publicationType |
Journal Article
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