Linked Life Data

16519896

Source:http://linkedlifedata.com/resource/pubmed/id/16519896

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2006-3-27
pubmed:abstractText
Given the well-defined role of LIM-motif containing proteins in cytoskeletal organization, cell fate, and differentiation, we hypothesized that the regulation of LIM proteins played an integral role in vascular remodeling. We screened a compendium of cDNA microarray data from rat vascular smooth muscle cells (VSMC) for novel LIM-containing targets and identified muscle LIM protein (MLP), a gene previously thought to be only in striated muscle. Sequence analysis, RTQPCR and Western blotting reconfirmed expression of MLP in VSMC. MLP was elevated>10-fold 7 days following balloon injury in the rat carotid artery. Wire injury led to a significantly increased intima/media ratio in MLP -/- mice compared to wild-type controls (P<0.007, N=5). Fas-ligand and ceramide-induced apoptosis were significantly decreased in MLP deficient VSMC (n=6, P<0.001). Adenoviral-induced restoration of MLP significantly restored apoptotic response (N=6, P<0.001). These findings are the first to identify MLP in vascular smooth muscle and demonstrate that it plays a critical role in vascular remodeling. This is consistent with earlier findings demonstrating a role for MLP in striated muscle remodeling in response to load and stretch.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-2828
pubmed:author
pubmed:issnType
Print
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
503-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:16519896-Animals, pubmed-meshheading:16519896-Aorta, pubmed-meshheading:16519896-Apoptosis, pubmed-meshheading:16519896-Carotid Artery Injuries, pubmed-meshheading:16519896-Cell Differentiation, pubmed-meshheading:16519896-Cells, Cultured, pubmed-meshheading:16519896-Cytoskeleton, pubmed-meshheading:16519896-Gene Expression Regulation, pubmed-meshheading:16519896-Intracellular Signaling Peptides and Proteins, pubmed-meshheading:16519896-Membrane Proteins, pubmed-meshheading:16519896-Mice, pubmed-meshheading:16519896-Mice, Knockout, pubmed-meshheading:16519896-Muscle, Skeletal, pubmed-meshheading:16519896-Myocytes, Smooth Muscle, pubmed-meshheading:16519896-Rats, pubmed-meshheading:16519896-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:16519896-Vesicular Transport Proteins
pubmed:year
2006
pubmed:articleTitle
A role for muscle LIM protein (MLP) in vascular remodeling.
pubmed:affiliation
Lillehei Heart Institute, Division of Cardiology, Department of Medicine, University of Minnesota, 420 Delaware Street SE, Mayo Mail Code 508, Minneapolis, MN 55455, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't