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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1991-9-20
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pubmed:abstractText |
Human cell lines producing monoclonal antibodies (MAbs) against Pseudomonas aeruginosa exotoxin A were established by EBV transformation followed by cell fusion. Monoclonal antibody FK-001, IgM (mu, kappa), was demonstrated to be specifically reactive with exotoxin A in ELISA and immunoblotting, by recognizing N-terminal 16 amino acid residues of exotoxin A as an epitope. This epitope region belongs to domain I which is required for the binding of exotoxin A to the receptor on target cells. FK-001 showed a partial neutralizing activity for cell toxicity caused by exotoxin A and appeared to be effective against exotoxin A-producing P. aeruginosa infection in mice. A line of evidence suggests that monoclonal antibody FK-001 neutralizes exotoxin A-induced cell toxicity by the interference of accessibility and/or binding of exotoxin A to animal cell receptors.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0272-457X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
297-307
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:1651902-ADP Ribose Transferases,
pubmed-meshheading:1651902-Animals,
pubmed-meshheading:1651902-Antibodies, Monoclonal,
pubmed-meshheading:1651902-Bacterial Toxins,
pubmed-meshheading:1651902-Cell Fusion,
pubmed-meshheading:1651902-Cell Line, Transformed,
pubmed-meshheading:1651902-Exotoxins,
pubmed-meshheading:1651902-Herpesvirus 4, Human,
pubmed-meshheading:1651902-Humans,
pubmed-meshheading:1651902-Immunization, Passive,
pubmed-meshheading:1651902-Mice,
pubmed-meshheading:1651902-Mice, Inbred BALB C,
pubmed-meshheading:1651902-Neutralization Tests,
pubmed-meshheading:1651902-Pseudomonas Infections,
pubmed-meshheading:1651902-Pseudomonas aeruginosa,
pubmed-meshheading:1651902-Rabbits,
pubmed-meshheading:1651902-Virulence Factors
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pubmed:year |
1991
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pubmed:articleTitle |
Production and characterization of human monoclonal antibody recognizing the N-terminal residues of Pseudomonas aeruginosa exotoxin A.
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pubmed:affiliation |
Laboratory of Biotechnology, Takarazuka Research Center, Sumitomo Chemical Co., Ltd., Hyogo, Japan.
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pubmed:publicationType |
Journal Article
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