|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
2006-3-6
|
|
pubmed:abstractText |
Clostridium perfringens strains (type A) isolated from an integrated poultry operation were subtyped using repetitive-element PCR with Dt primers. Isolates were obtained from fecal, egg shell, fluff, and carcass rinse samples as part of a previously reported temporally linked epidemiological survey. A total of 48 isolates of C. perfringens were obtained from different stages of the broiler chicken production chain from two separate breeder farms that supplied a single hatchery that in turn provided chicks to a single grow-out farm whose flocks were processed at a single plant. All 48 isolates were typeable (100% typeability) by repetitive-element PCR with Dt primers. This subtyping method was highly reproducible and discriminatory. By repetitive-element PCR with Dt primers, isolates were classified into four major branches with 12 subgroups or clades. The Simpson's index of discrimination was calculated to be 0.96 for groupings of >95% correlation. Toxin gene profiles of the isolates indicated that all of the isolates were C. perfringens alpha-toxin gene positive and 46 of 48 isolates were beta2-toxin gene positive. All strains were negative for beta- and epsilon-toxin genes. Repetitive sequence-based PCR was found to be a technically practical and reproducible means of subtyping C. perfringens libraries from specific epidemiological or production environment settings.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-10203838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-10224001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-10325304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-10357280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-10364587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-10397312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-10397320,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-10443537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-10511517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-10857923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-11230399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-11770623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-11940151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-11981970,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-12144198,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-12147468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-12574310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-12620854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-12791864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-12791907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-12814890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-12814893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-12837737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-14557031,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-14562900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-1488953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-15128519,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-15634972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-16000804,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-1624445,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-1762913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-1777387,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-2542360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-3069867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-6089664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-63553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-7989550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-8074510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-8082823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-8349778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-8679144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-8727902,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-8964036,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-8968913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-8971615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-9003592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-9215442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-9327563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-9708289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-9728690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-9797260,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517895-9926457
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
0095-1137
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
44
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1065-73
|
|
pubmed:dateRevised |
2010-9-15
|
|
pubmed:meshHeading |
pubmed-meshheading:16517895-Animals,
pubmed-meshheading:16517895-Bacterial Toxins,
pubmed-meshheading:16517895-Bacterial Typing Techniques,
pubmed-meshheading:16517895-Calcium-Binding Proteins,
pubmed-meshheading:16517895-Chickens,
pubmed-meshheading:16517895-Clostridium perfringens,
pubmed-meshheading:16517895-DNA, Bacterial,
pubmed-meshheading:16517895-Food Microbiology,
pubmed-meshheading:16517895-Genes, Bacterial,
pubmed-meshheading:16517895-Polymerase Chain Reaction,
pubmed-meshheading:16517895-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:16517895-Type C Phospholipases
|
|
pubmed:year |
2006
|
|
pubmed:articleTitle |
Molecular subtyping of poultry-associated type A Clostridium perfringens isolates by repetitive-element PCR.
|
|
pubmed:affiliation |
Agricultural Research Service, United States Department of Agriculture, Russell Research Center, Athens, Georgia 30604-5677, USA. siragusa@saa.ars.usda.gov
|
