Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2006-3-6
pubmed:abstractText
African swine fever (ASF) is an infectious and economically important disease of domestic pigs. The absence of vaccine renders the diagnostic test the only tool that can be used for the control of new outbreaks of the disease. At present, the enzyme-linked immunosorbent assay (ELISA) test is the most useful method for large-scale ASF serological studies, although false positives have been detected, mainly on poorly preserved sera. In order to improve the current diagnostic test available for ASF, we have studied the antigenic properties of the ASF virus polyprotein pp62 and its suitability for use in a novel ELISA. Two well-known antigenic proteins of ASF virus, p32 and p54, were also included in this study. These proteins were expressed in the baculovirus expression system and used as antigens in ASF serological tests. Our results indicate that the use of these recombinant proteins as antigens in the ELISAs improves the sensitivity and specificity obtained with the conventional diagnosis test used to detect antibodies against ASF virus. Furthermore, the use of polyprotein pp62 in an ELISA for testing poorly preserved sera allows performance of the diagnosis of ASF without the need to confirm the results by the immunoblot test. These features make pp62 one of the most interesting viral proteins to be used for serological ASF diagnosis.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16517882-11831707, http://linkedlifedata.com/resource/pubmed/commentcorrection/16517882-11922604, http://linkedlifedata.com/resource/pubmed/commentcorrection/16517882-12438573, http://linkedlifedata.com/resource/pubmed/commentcorrection/16517882-1317266, http://linkedlifedata.com/resource/pubmed/commentcorrection/16517882-1376539, http://linkedlifedata.com/resource/pubmed/commentcorrection/16517882-15047843, http://linkedlifedata.com/resource/pubmed/commentcorrection/16517882-1515486, http://linkedlifedata.com/resource/pubmed/commentcorrection/16517882-2094444, http://linkedlifedata.com/resource/pubmed/commentcorrection/16517882-2672914, http://linkedlifedata.com/resource/pubmed/commentcorrection/16517882-2722819, http://linkedlifedata.com/resource/pubmed/commentcorrection/16517882-2914218, http://linkedlifedata.com/resource/pubmed/commentcorrection/16517882-2916332, http://linkedlifedata.com/resource/pubmed/commentcorrection/16517882-3521071, http://linkedlifedata.com/resource/pubmed/commentcorrection/16517882-3776350, http://linkedlifedata.com/resource/pubmed/commentcorrection/16517882-3989907, http://linkedlifedata.com/resource/pubmed/commentcorrection/16517882-4950035, http://linkedlifedata.com/resource/pubmed/commentcorrection/16517882-6168100, http://linkedlifedata.com/resource/pubmed/commentcorrection/16517882-7769024, http://linkedlifedata.com/resource/pubmed/commentcorrection/16517882-7933107, http://linkedlifedata.com/resource/pubmed/commentcorrection/16517882-8074873, http://linkedlifedata.com/resource/pubmed/commentcorrection/16517882-8236717, http://linkedlifedata.com/resource/pubmed/commentcorrection/16517882-8764090, http://linkedlifedata.com/resource/pubmed/commentcorrection/16517882-9029527, http://linkedlifedata.com/resource/pubmed/commentcorrection/16517882-9223468, http://linkedlifedata.com/resource/pubmed/commentcorrection/16517882-9568043
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
44
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
950-6
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Antigenic properties and diagnostic potential of African swine fever virus protein pp62 expressed in insect cells.
pubmed:affiliation
CISA-INIA, Carretera Algete a El Casar, sn. Valdeolmos, Madrid 28130, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Evaluation Studies