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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1991-9-25
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pubmed:abstractText |
To optimize growth and Ig production of in vitro-cultured Epstein-Barr virus (EBV)-transformed B cells, a panel of six monoclonal EBV B-cell lines was analyzed for autocrine growth factor production and responsiveness to various cytokines. Three cell lines produced Il-I and four produced Il-6, although differences concerning the amount of lymphokines produced were observed. Interestingly a considerable tumor necrosis factor beta (lymphotoxin) activity was found in supernatants of all cell lines. One IgM-producing cell line that did not secrete either Il-1 or Il-6 was exceptional in its ability to respond to the addition of rIl-6 with a 5- to 10-times elevated IgM production. In contrast, cell lines in the panel capable of Il-6 production showed only a minimal elevation of Ig production on addition of exogenous Il-6. Ig production was slightly less in some cell lines when Il-6 was neutralized. Antibodies against lymphotoxin or Il-6 did not influence growth rate of the cell lines significantly, implying that neither Il-6 nor lymphotoxin had an autostimulatory effect on the analyzed cell lines. This study demonstrates a heterogeneity regarding the amount and type of lymphokines produced by long-term monoclonal EBV cell lines, which may account for the diverse responses exhibited by these cells towards exogenously added lymphokines.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0956-960X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
57-64
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1651786-B-Lymphocytes,
pubmed-meshheading:1651786-Cell Differentiation,
pubmed-meshheading:1651786-Cell Division,
pubmed-meshheading:1651786-Cell Line, Transformed,
pubmed-meshheading:1651786-Cell Transformation, Viral,
pubmed-meshheading:1651786-Herpesvirus 4, Human,
pubmed-meshheading:1651786-Humans,
pubmed-meshheading:1651786-Interleukin-6,
pubmed-meshheading:1651786-Lymphokines,
pubmed-meshheading:1651786-Lymphotoxin-alpha,
pubmed-meshheading:1651786-Tumor Necrosis Factor-alpha
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pubmed:year |
1991
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pubmed:articleTitle |
Heterogeneity in both cytokine production and responsiveness of a panel of monoclonal human Epstein-Barr virus-transformed B-cell lines.
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pubmed:affiliation |
Department of Hybridoma and Tissue Culture, Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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