Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1991-9-19
pubmed:abstractText
Polyomavirus late mRNAs contain multiple copies of a 57-nucleotide leader sequence derived by splicing from multigenome-length late transcripts. Inefficient termination of transcription and inefficient polyadenylation allow accumulation of these giant transcripts. In this report, we show that a viable mutant virus, ins5, which contains an efficient rabbit beta-globin polyadenylation signal, produced late mRNAs whose vast majority contains only one leader. ins5 virus nevertheless produced as much late mRNA as did wild-type virus and grew as well as did wild-type virus in mouse cells. These results demonstrate that leader-to-leader splicing per se is not required for efficient production of late mRNAs or for efficient virus replication. However, we also found that RNAs lacking critical sequences near the 5' end of the leader did not accumulate as mRNAs and that most late transcripts made during the early part of the late phase, when few late mRNAs are produced, initiated downstream of the 5' end of the leader. These results indicate that a sequence element near the 5' end of the leader is required for proper processing, transport, or stability of late mRNAs and that the control of late mRNA production depends in part on the choice of transcription initiation sites at the late promoter.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1651405-173883, http://linkedlifedata.com/resource/pubmed/commentcorrection/1651405-215784, http://linkedlifedata.com/resource/pubmed/commentcorrection/1651405-2173769, http://linkedlifedata.com/resource/pubmed/commentcorrection/1651405-222459, http://linkedlifedata.com/resource/pubmed/commentcorrection/1651405-229965, http://linkedlifedata.com/resource/pubmed/commentcorrection/1651405-2535755, http://linkedlifedata.com/resource/pubmed/commentcorrection/1651405-2542572, http://linkedlifedata.com/resource/pubmed/commentcorrection/1651405-2547989, http://linkedlifedata.com/resource/pubmed/commentcorrection/1651405-2550672, http://linkedlifedata.com/resource/pubmed/commentcorrection/1651405-2824837, http://linkedlifedata.com/resource/pubmed/commentcorrection/1651405-2836265, http://linkedlifedata.com/resource/pubmed/commentcorrection/1651405-2846852, http://linkedlifedata.com/resource/pubmed/commentcorrection/1651405-2847921, http://linkedlifedata.com/resource/pubmed/commentcorrection/1651405-2848251, http://linkedlifedata.com/resource/pubmed/commentcorrection/1651405-2871197, http://linkedlifedata.com/resource/pubmed/commentcorrection/1651405-2985970, http://linkedlifedata.com/resource/pubmed/commentcorrection/1651405-3009856, http://linkedlifedata.com/resource/pubmed/commentcorrection/1651405-3018549, http://linkedlifedata.com/resource/pubmed/commentcorrection/1651405-3023898, http://linkedlifedata.com/resource/pubmed/commentcorrection/1651405-3024968, http://linkedlifedata.com/resource/pubmed/commentcorrection/1651405-3031605, http://linkedlifedata.com/resource/pubmed/commentcorrection/1651405-3479794, http://linkedlifedata.com/resource/pubmed/commentcorrection/1651405-6095108, http://linkedlifedata.com/resource/pubmed/commentcorrection/1651405-6243401, http://linkedlifedata.com/resource/pubmed/commentcorrection/1651405-6251239, http://linkedlifedata.com/resource/pubmed/commentcorrection/1651405-6255435, http://linkedlifedata.com/resource/pubmed/commentcorrection/1651405-6270372, http://linkedlifedata.com/resource/pubmed/commentcorrection/1651405-6271970, http://linkedlifedata.com/resource/pubmed/commentcorrection/1651405-6275357, http://linkedlifedata.com/resource/pubmed/commentcorrection/1651405-6292484, http://linkedlifedata.com/resource/pubmed/commentcorrection/1651405-6325893
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
65
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4728-34
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Production of polyomavirus late mRNAs requires sequences near the 5' end of the leader but does not require leader-to-leader splicing.
pubmed:affiliation
Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't