Linked Life Data

16513897

Source:http://linkedlifedata.com/resource/pubmed/id/16513897

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2006-5-23
pubmed:abstractText
Maturation of fetal alveolar type II epithelial cells in utero is characterized by specific changes to lung surfactant phospholipids. Here, we quantified the effects of hormonal differentiation in vitro on the molecular specificity of cellular and secreted phospholipids from human fetal type II epithelial cells using electrospray ionization mass spectrometry. Differentiation, assessed by morphology and changes in gene expression, was accompanied by restricted and specific modifications to cell phospholipids, principally enrichments of shorter chain species of phosphatidylcholine (PC) and phosphatidylinositol, that were not observed in fetal lung fibroblasts. Treatment of differentiated epithelial cells with secretagogues stimulated the secretion of functional surfactant-containing surfactant proteins B and C (SP-B and SP-C). Secreted material was further enriched in this same set of phospholipid species but was characterized by increased contents of short-chain monounsaturated and disaturated species other than dipalmitoyl PC (PC16:0/16:0), principally palmitoylmyristoyl PC (PC16:0/14:0) and palmitoylpalmitoleoyl PC (PC16:0/16:1). Mixtures of these PC molecular species, phosphatidylglycerol, and SP-B and SP-C were functionally active and rapidly generated low surface tension on compression in a pulsating bubble surfactometer. These results suggest that hormonally differentiated human fetal type II cells do not select the molecular composition of surfactant phospholipid on the basis of saturation but, more likely, on the basis of acyl chain length.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0022-2275
pubmed:author
pubmed:issnType
Print
pubmed:volume
47
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1322-31
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:16513897-1,2-Dipalmitoylphosphatidylcholine, pubmed-meshheading:16513897-1-Methyl-3-isobutylxanthine, pubmed-meshheading:16513897-8-Bromo Cyclic Adenosine Monophosphate, pubmed-meshheading:16513897-Cell Differentiation, pubmed-meshheading:16513897-Cells, Cultured, pubmed-meshheading:16513897-Dexamethasone, pubmed-meshheading:16513897-Dimyristoylphosphatidylcholine, pubmed-meshheading:16513897-Epithelial Cells, pubmed-meshheading:16513897-Female, pubmed-meshheading:16513897-Humans, pubmed-meshheading:16513897-Lipid Metabolism, pubmed-meshheading:16513897-Phosphatidylcholines, pubmed-meshheading:16513897-Phospholipids, pubmed-meshheading:16513897-Pulmonary Alveoli, pubmed-meshheading:16513897-Pulmonary Surfactant-Associated Proteins, pubmed-meshheading:16513897-Pulmonary Surfactants, pubmed-meshheading:16513897-Surface Tension
pubmed:year
2006
pubmed:articleTitle
Lipidomics of cellular and secreted phospholipids from differentiated human fetal type II alveolar epithelial cells.
pubmed:affiliation
Division of Infection, Inflammation, and Repair, School of Medicine, University of Southampton, Southampton, UK. adp@soton.ac.uk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural