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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1991-9-17
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pubmed:abstractText |
The production of superoxide anion (O2-) by phagocytic cells plays an important role in host defenses and inflammatory processes. Interferon-gamma (IFN-gamma) primes neutrophils for increased O2- release stimulated by various agonists. This study examines if myeloid differentiated HL-60 cells also serve as a model for IFN-gamma-induced priming, and examines mechanism by which this priming occurs. IFN-gamma enhanced HL-60 cell superoxide production in response to F-Met-Leu-Phe (FMLP) in a concentration-dependent manner. Following a 4-h exposure, an increase in O2- production was seen with IFN-gamma at 0.1 U/ml, with optimal priming at 100 U/ml. The time course of priming by 100 U/ml IFN-gamma showed that at least a 1-h exposure was required, and a maximal effect was seen at 24 h. Priming after a 4-h exposure to 100 U/ml IFN-gamma was completely inhibited by 1 micrograms/ml cycloheximide. HL-60 cells cultivated with 100 U/ml IFN-gamma produced increased O2- when exposed to 25 mM NaF (containing AIF4) or 10 nM phorbol myristate acetate, agonists that trigger the respiratory burst independent of receptor stimulation. These results indicate that IFN-gamma primes the HL-60 cell respiratory burst in a concentration and time-dependent manner similar to its effect on neutrophils. The data are consistent with the hypothesis that IFN-gamma primes HL-60 cells, in part, by stimulating synthesis of proteins that participate in NADPH oxidase activation distal to the FMLP receptor.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/N-Formylmethionine...,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium Fluoride,
http://linkedlifedata.com/resource/pubmed/chemical/Superoxides,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0197-8357
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
69-74
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:1651364-Antigens, Surface,
pubmed-meshheading:1651364-Cell Differentiation,
pubmed-meshheading:1651364-Humans,
pubmed-meshheading:1651364-Interferon-gamma,
pubmed-meshheading:1651364-Models, Biological,
pubmed-meshheading:1651364-N-Formylmethionine Leucyl-Phenylalanine,
pubmed-meshheading:1651364-Neutrophils,
pubmed-meshheading:1651364-Protein Biosynthesis,
pubmed-meshheading:1651364-Sodium Fluoride,
pubmed-meshheading:1651364-Superoxides,
pubmed-meshheading:1651364-Tetradecanoylphorbol Acetate,
pubmed-meshheading:1651364-Tumor Cells, Cultured
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pubmed:year |
1991
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pubmed:articleTitle |
Interferon-gamma enhances superoxide production by HL-60 cells stimulated with multiple agonists.
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pubmed:affiliation |
Department of Medicine, University of Louisville School of Medicine, KY.
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pubmed:publicationType |
Journal Article
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