|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0010423,
umls-concept:C0017262,
umls-concept:C0033684,
umls-concept:C0036488,
umls-concept:C0043309,
umls-concept:C0185117,
umls-concept:C0216706,
umls-concept:C0439611,
umls-concept:C0936012,
umls-concept:C1514562,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221,
umls-concept:C1998793,
umls-concept:C2911684
|
|
pubmed:issue |
Pt 1
|
|
pubmed:dateCreated |
2006-3-2
|
|
pubmed:abstractText |
A family of animal proteins is emerging which contain a conserved protein motif known as an olfactomedin (OLF) domain. Novel extracellular protein-protein interactions occur through this domain. The OLF-family member amassin, from the sea urchin Strongylocentrotus purpuratus, has previously been identified to mediate a rapid cell-adhesion event resulting in a large aggregation of coelomocytes, the circulating immune cells. In this work, heterologous expression and purification of the OLF domain from amassin was carried out and initial crystallization trials were performed. A native data set has been collected, extending to 2.7 A under preliminary cryoconditions, using an in-house generator. This work leads the way to the determination of the first structure of an OLF domain.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511251-10221915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511251-10570136,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511251-10783240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511251-10842733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511251-10860755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511251-11558819,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511251-11567148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511251-11752314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511251-11784068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511251-11856527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511251-12019210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511251-12591917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511251-12615070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511251-14619973,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511251-14993666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511251-14999070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511251-15136047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511251-15299374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511251-16039564,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511251-1892825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511251-5700707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511251-8506313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511251-9188456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511251-9328473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511251-9790844
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jan
|
|
pubmed:issn |
1744-3091
|
|
pubmed:author |
|
|
pubmed:issnType |
Electronic
|
|
pubmed:day |
1
|
|
pubmed:volume |
62
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
16-9
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:16511251-Animals,
pubmed-meshheading:16511251-Cell Adhesion Molecules,
pubmed-meshheading:16511251-Crystallization,
pubmed-meshheading:16511251-Crystallography, X-Ray,
pubmed-meshheading:16511251-Escherichia coli,
pubmed-meshheading:16511251-Extracellular Matrix Proteins,
pubmed-meshheading:16511251-Glycoproteins,
pubmed-meshheading:16511251-Protein Structure, Tertiary,
pubmed-meshheading:16511251-Recombinant Proteins,
pubmed-meshheading:16511251-Strongylocentrotus purpuratus
|
|
pubmed:year |
2006
|
|
pubmed:articleTitle |
Expression, purification, crystallization and preliminary X-ray analysis of the olfactomedin domain from the sea urchin cell-adhesion protein amassin.
|
|
pubmed:affiliation |
Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California San Diego, La Jolla, CA 92093-0202, USA. bhillier@ucsd.edu
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|
