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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
Pt 3
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pubmed:dateCreated |
2006-3-2
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pubmed:abstractText |
The class I CD8 positive T-cell response is involved in a number of conditions in which artificial down-regulation and control would be therapeutically beneficial. Such conditions include a number of autoimmune diseases and graft rejection in transplant patients. Although the CD8 T-cell response is dominated by the TCR-pMHC interaction, activation of T cells is in most cases also dependent on a number of associated signalling molecules. Previous work has demonstrated the ability of one such molecule (CD8) to act as an antagonist to T-cell activation if added in soluble form. Therefore, a high-affinity mutant CD8 (haCD8) has been developed with the aim of developing a therapeutic immunosuppressor. In order to fully understand the nature of the haCD8 interaction, this protein was crystallized using the sitting-drop vapour-diffusion method. Single haCD8 crystals were cryocooled and used for data collection. These crystals belonged to space group P6(4)22 (assumed by similarity to the wild type), with unit-cell parameters a = 101.08, c = 56.54 A. VM calculations indicated one molecule per asymmetric unit. A 2 A data set was collected and the structure is currently being determined using molecular replacement.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511019-10202928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511019-10430939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511019-10657671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511019-10934227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511019-10963600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511019-11986610,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511019-12144809,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511019-15299374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511019-1534242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511019-2509117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511019-3031507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511019-3043226,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511019-3315856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511019-5133731,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511019-6975943,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511019-6981845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511019-8127870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511019-8638282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511019-9177355,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511019-9605330,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511019-9806633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16511019-9809559
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1744-3091
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pubmed:author |
pubmed-author:BellJohn IJI,
pubmed-author:BoulterJonathan MJM,
pubmed-author:ColeDavid KDK,
pubmed-author:GaoFengF,
pubmed-author:GaoGeorge FGF,
pubmed-author:GlickMeirM,
pubmed-author:JakobsenBent KBK,
pubmed-author:LissinNikolai MNM,
pubmed-author:RizkallahPierre JPJ,
pubmed-author:SamiMalkitM,
pubmed-author:VuidepotAnne LiseAL
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
61
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
285-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:16511019-CD8-Positive T-Lymphocytes,
pubmed-meshheading:16511019-Cloning, Molecular,
pubmed-meshheading:16511019-Crystallization,
pubmed-meshheading:16511019-Humans,
pubmed-meshheading:16511019-Major Histocompatibility Complex,
pubmed-meshheading:16511019-Protein Conformation,
pubmed-meshheading:16511019-Protein Denaturation,
pubmed-meshheading:16511019-Protein Folding,
pubmed-meshheading:16511019-Receptors, Antigen, T-Cell,
pubmed-meshheading:16511019-Recombinant Proteins
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pubmed:year |
2005
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pubmed:articleTitle |
Crystallization and preliminary X-ray structural studies of a high-affinity CD8alphaalpha co-receptor to pMHC.
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pubmed:affiliation |
Nuffield Department of Clinical Medicine, John Radcliffe Hospital, Oxford University, Oxford OX3 9DU, England.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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