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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
2006-7-19
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pubmed:abstractText |
The majority of the neutralizing epitopes of papillomaviruses (PV) are conformation-specific and have not been fully characterised. Studies have, to date, been limited to a few HPV types only. We analysed the epitopes on the major capsid protein (L1) of Human papillomavirus (HPV) type 31 using monoclonal antibodies (MAbs) generated against HPV-31 virus-like particles (VLPs). The type-specific MAbs against HPV-31 were all found to be neutralizing and recognized conformation-dependent epitopes. Two other MAbs directed against a conformational epitope were found to be cross-reactive with other HPV types, and one of them was found to be cross-neutralizing. Cross-reactive antibodies were further investigated using wild-type HPV-16 L1 VLPs and two mutants. The results obtained suggested the existence of a cross-neutralizing conformational epitope at the N-terminal part of the FG loop of the major capsid protein, and the other four cross-reactive MAbs recognized epitopes also located at the N-terminal part of the FG loop.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Capsid Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/HPV L1 protein, Human papillomavirus,
http://linkedlifedata.com/resource/pubmed/chemical/L2 protein, Human papillomavirus...,
http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Proteins, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0304-8608
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
151
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1511-23
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16508703-Amino Acid Sequence,
pubmed-meshheading:16508703-Animals,
pubmed-meshheading:16508703-Antibodies, Monoclonal,
pubmed-meshheading:16508703-Capsid Proteins,
pubmed-meshheading:16508703-Cells, Cultured,
pubmed-meshheading:16508703-Cross Reactions,
pubmed-meshheading:16508703-Epitope Mapping,
pubmed-meshheading:16508703-Epitopes,
pubmed-meshheading:16508703-Female,
pubmed-meshheading:16508703-Humans,
pubmed-meshheading:16508703-Mice,
pubmed-meshheading:16508703-Mice, Inbred BALB C,
pubmed-meshheading:16508703-Molecular Sequence Data,
pubmed-meshheading:16508703-Neutralization Tests,
pubmed-meshheading:16508703-Oncogene Proteins, Viral,
pubmed-meshheading:16508703-Papillomaviridae,
pubmed-meshheading:16508703-Peptides,
pubmed-meshheading:16508703-Protein Conformation,
pubmed-meshheading:16508703-Spodoptera,
pubmed-meshheading:16508703-Virion
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pubmed:year |
2006
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pubmed:articleTitle |
Identification of type-specific and cross-reactive neutralizing conformational epitopes on the major capsid protein of human papillomavirus type 31.
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pubmed:affiliation |
INSERM U618, Université François Rabelais, Tours, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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