Linked Life Data

16507746

Source:http://linkedlifedata.com/resource/pubmed/id/16507746

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2006-3-1
pubmed:abstractText
In vitro experimentation is valuable as a precursor to in vivo research; however, as we all know too well, not every in vitro finding leads to confirmation in vivo. In fact, conflicting results often occur because in vitro experiments lack all the necessary factors that impinge upon normal physiology in live animals. We are fortunate when in vivo models can be created that allow for direct observation of pharmacological effects. Kaelin and colleagues have created a mouse line that expresses the oxygen-dependent degradation domain (ODD) of hypoxia-induced factor-1alpha (HIF-1alpha) fused to the common firefly luciferase gene, under the control of a promoter that ensures organism-wide expression. In conditions of normoxia, the protein is quickly degraded, but in hypoxic situations, the protein is not degraded and administration of substrate illuminates the areas of expression. This technique allows for the observation of the effects of agents on oxygen tension in particular organs. Implicit is the adaptability of this model, wherein other promoters (e.g., tissue-specific) can be utilized or other disease models can be studied.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1534-0384
pubmed:author
pubmed:issnType
Print
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
20-2, 2
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Lights on for low oxygen: a noninvasive mouse model useful for sensing oxygen deficiency.
pubmed:affiliation
Laboratory of Cancer Prevention, National Cancer Institute-Frederick, Maryland 21702, USA. Tschmid@ncifcrf.gov
pubmed:publicationType
Journal Article, Research Support, N.I.H., Intramural