Linked Life Data

165069

Source:http://linkedlifedata.com/resource/pubmed/id/165069

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1975-8-8
pubmed:abstractText
Differences exist in the rates at which hormones are inactivated by, or dissociate from, their target tissues. The present studies examined the binding of biologically active TSH to thyroid slices and compared its characteristics to those of PGE. Canine thyroid slices were initally incubated with 5 mU/ML OF BOVINE TSH (TSH-Inital) for 15 min, washed and incubated in media free of hormone for 3 hr. At the conclusion of this second incubation period all slices were again washed. Some were then transferred to media containing 10-2M theophylline for a final 10 min incubation and subsequent measurement of cAMP and protein kinase, while others were transferred to media containing (l-14C)glucose without theophylline for a final 45 min incubation to assess glucose oxidation. Identically treated slices never exposed to TSH served as controls, while others were exposed to TSH only during the final 10 or 45 min incubation periods (TSH-Final). cAMP content determined after significantly increased in TSH-Initial (mean 2.98 plus or minus 0.36 (se) pmol/mg wet wt) compared to control (0.35 plus or minus 0.04), but was less than that in TSH-Final (5.76 plus or minus 0.51). This phenomenon was not unique to canine thyroid, since comparable results were noted in studies of human, bovine or porcine thyroid slices. The protein kinase activity ratio (-cAMP/+cAMP) and glucose oxidation of TSH-Initial were also significantly increased above control following the final 10 min or 45 min incubations respectively. Addition of trypsin to the 3 h incubation abolished the subsequent increase in cAMP in TSH-Initial, while addition of TSH antiserum appreciably reduced this increase. These results are consistent with the persistent binding of biologically active TSH to thyroid. By contrast, evidence of similar persistent binding of PGE1 to thyroid, glucagon to liver, or parathyroid hormone to renal cortex was lacking when assessed by an identical experimental procedure. Differences between the duration of interaction of TSH and PGE1 with thyroid may be dependent or a more gradual dissociation to tissue bound TSH, a more rapid inactivation of bound-PGE1, or both.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
96
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1579-86
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:165069-Animals, pubmed-meshheading:165069-Antigen-Antibody Reactions, pubmed-meshheading:165069-Binding Sites, pubmed-meshheading:165069-Cattle, pubmed-meshheading:165069-Cyclic AMP, pubmed-meshheading:165069-Dogs, pubmed-meshheading:165069-Glucose, pubmed-meshheading:165069-Humans, pubmed-meshheading:165069-Immune Sera, pubmed-meshheading:165069-Kidney Cortex, pubmed-meshheading:165069-Kinetics, pubmed-meshheading:165069-Liver, pubmed-meshheading:165069-Organ Specificity, pubmed-meshheading:165069-Receptors, Cell Surface, pubmed-meshheading:165069-Species Specificity, pubmed-meshheading:165069-Swine, pubmed-meshheading:165069-Theophylline, pubmed-meshheading:165069-Thyroid Gland, pubmed-meshheading:165069-Thyrotropin, pubmed-meshheading:165069-Time Factors, pubmed-meshheading:165069-Trypsin
pubmed:year
1975
pubmed:articleTitle
Evidence for persistent binding of biologically active thyrotropin to thyroid in vitro.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.