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rdf:type |
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lifeskim:mentions |
umls-concept:C0003577,
umls-concept:C0017262,
umls-concept:C0027882,
umls-concept:C0028608,
umls-concept:C0185117,
umls-concept:C0205263,
umls-concept:C0234112,
umls-concept:C0443252,
umls-concept:C1414236,
umls-concept:C1415090,
umls-concept:C1514873,
umls-concept:C1546857,
umls-concept:C1556066,
umls-concept:C1619636,
umls-concept:C1837550,
umls-concept:C2911684
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pubmed:issue |
5
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pubmed:dateCreated |
2006-2-28
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|
pubmed:abstractText |
In Aplysia, long-term synaptic plasticity is induced by serotonin (5-HT) or neural activity and requires gene expression. Here, we demonstrate that ApLLP, a novel nucleolus protein, is critically involved in both long-term facilitation (LTF) and behavioral sensitization. Membrane depolarization induced ApLLP expression, which activated ApC/EBP expression through a direct binding to CRE. LTF was produced by a single pulse of 5-HT 30 min after the membrane depolarization. This LTF was blocked when either ApLLP or ApC/EBP were blocked by specific antibodies. In contrast, ApLLP overexpression induced LTF in response to a single 5-HT treatment. Simultaneously, a siphon noxious stimulus (SNS) to intact Aplysia induced ApLLP and ApC/EBP expression, and single tail shock 30 min after SNS transformed short-term sensitization to long-term sensitization of siphon withdrawal reflex. These results suggest that ApLLP is an activity-dependent transcriptional activator that switches short-term facilitation to long-term facilitation.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
|
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0896-6273
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
2
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pubmed:volume |
49
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pubmed:owner |
NLM
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|
pubmed:authorsComplete |
Y
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pubmed:pagination |
707-18
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:16504946-Analysis of Variance,
pubmed-meshheading:16504946-Animals,
pubmed-meshheading:16504946-Aplysia,
pubmed-meshheading:16504946-Avoidance Learning,
pubmed-meshheading:16504946-Behavior, Animal,
pubmed-meshheading:16504946-Blotting, Western,
pubmed-meshheading:16504946-CCAAT-Enhancer-Binding Proteins,
pubmed-meshheading:16504946-Cells, Cultured,
pubmed-meshheading:16504946-Dose-Response Relationship, Drug,
pubmed-meshheading:16504946-Electric Stimulation,
pubmed-meshheading:16504946-Electrophoretic Mobility Shift Assay,
pubmed-meshheading:16504946-Gene Expression,
pubmed-meshheading:16504946-Gene Expression Regulation,
pubmed-meshheading:16504946-Green Fluorescent Proteins,
pubmed-meshheading:16504946-Immunohistochemistry,
pubmed-meshheading:16504946-In Situ Hybridization,
pubmed-meshheading:16504946-Long-Term Potentiation,
pubmed-meshheading:16504946-Microinjections,
pubmed-meshheading:16504946-Models, Biological,
pubmed-meshheading:16504946-Neurons,
pubmed-meshheading:16504946-Potassium,
pubmed-meshheading:16504946-Promoter Regions, Genetic,
pubmed-meshheading:16504946-RNA, Messenger,
pubmed-meshheading:16504946-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:16504946-Serotonin,
pubmed-meshheading:16504946-Time Factors,
pubmed-meshheading:16504946-Transcription Factors,
pubmed-meshheading:16504946-Transcriptional Activation
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|
pubmed:year |
2006
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|
pubmed:articleTitle |
A nucleolar protein ApLLP induces ApC/EBP expression required for long-term synaptic facilitation in aplysia neurons.
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|
pubmed:affiliation |
National Research Laboratory, Institute of Molecular Biology and Genetics, School of Biological Sciences, College of Natural Sciences, Seoul National University, San 56-1 Silim-dong Kwanak-gu, Seoul 151-742, Korea.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|
