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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1991-9-4
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pubmed:abstractText |
The effect of adenosine on phosphoinositide hydrolysis was examined in 1321N1 human astrocytoma cells. Adenosine, L-N6-phenylisopropyladenosine (L-PIA), and 5'-(N-ethylcarboxamido)adenosine (NECA) inhibited histamine-stimulated accumulation of inositol phosphates in a concentration-dependent manner. The potency order of adenosine analogues for inhibition of inositol phosphate accumulation was L-PIA greater than adenosine greater than NECA, a finding indicating that A1-class adenosine receptors are involved in the inhibition. The reduction in inositol phosphate accumulation by L-PIA was blocked by an adenosine receptor antagonist, 8-phenyltheophylline. Stimulation of A1-class adenosine receptors inhibited isoproterenol-stimulated cyclic AMP accumulation as well as histamine-induced inositol phosphate accumulation. Both inhibitory effects were blocked by pretreatment of the cells with pertussis toxin [islet-activating protein (IAP)]. L-PIA also inhibited guanosine 5'-(gamma-thio)triphosphate (GTP gamma S)-stimulated accumulation of inositol phosphates in membrane preparations, and 8-phenyl-theophylline antagonized the inhibition. L-PIA could not inhibit GTP gamma S-induced accumulation of inositol phosphates in IAP-treated membranes. Gi/Go, purified from rabbit brain, inhibited GTP gamma S-stimulated accumulation of inositol phosphates in a concentration-dependent manner in membrane preparations. These results suggest that stimulation of A1-class adenosine receptors interacts with the IAP-sensitive G protein(s), resulting in the inhibitions of phospholipase C as well as adenylate cyclase in human astrocytoma cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine-5'-(N-ethylcarboxamide),
http://linkedlifedata.com/resource/pubmed/chemical/Adenylate Cyclase,
http://linkedlifedata.com/resource/pubmed/chemical/Adenylate Cyclase Toxin,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Guanosine 5'-O-(3-Thiotriphosphate),
http://linkedlifedata.com/resource/pubmed/chemical/Histamine,
http://linkedlifedata.com/resource/pubmed/chemical/Pertussis Toxin,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositols,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Purinergic,
http://linkedlifedata.com/resource/pubmed/chemical/Type C Phospholipases,
http://linkedlifedata.com/resource/pubmed/chemical/Vasodilator Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Virulence Factors, Bordetella
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0022-3042
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
57
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
963-9
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:1650398-Adenosine,
pubmed-meshheading:1650398-Adenosine-5'-(N-ethylcarboxamide),
pubmed-meshheading:1650398-Adenylate Cyclase,
pubmed-meshheading:1650398-Adenylate Cyclase Toxin,
pubmed-meshheading:1650398-Astrocytoma,
pubmed-meshheading:1650398-Cells, Cultured,
pubmed-meshheading:1650398-Cyclic AMP,
pubmed-meshheading:1650398-Dose-Response Relationship, Drug,
pubmed-meshheading:1650398-GTP-Binding Proteins,
pubmed-meshheading:1650398-Guanosine 5'-O-(3-Thiotriphosphate),
pubmed-meshheading:1650398-Histamine,
pubmed-meshheading:1650398-Humans,
pubmed-meshheading:1650398-Hydrolysis,
pubmed-meshheading:1650398-Pertussis Toxin,
pubmed-meshheading:1650398-Phosphatidylinositols,
pubmed-meshheading:1650398-Receptors, Purinergic,
pubmed-meshheading:1650398-Tumor Cells, Cultured,
pubmed-meshheading:1650398-Type C Phospholipases,
pubmed-meshheading:1650398-Vasodilator Agents,
pubmed-meshheading:1650398-Virulence Factors, Bordetella
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pubmed:year |
1991
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pubmed:articleTitle |
Adenosine inhibits histamine-induced phosphoinositide hydrolysis mediated via pertussis toxin-sensitive G protein in human astrocytoma cells.
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pubmed:affiliation |
Department of Pharmacology, Fukushima Medical College, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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