| pubmed-article:1650372 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1650372 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:1650372 | lifeskim:mentions | umls-concept:C0032043 | lld:lifeskim |
| pubmed-article:1650372 | lifeskim:mentions | umls-concept:C1167207 | lld:lifeskim |
| pubmed-article:1650372 | lifeskim:mentions | umls-concept:C1135936 | lld:lifeskim |
| pubmed-article:1650372 | lifeskim:mentions | umls-concept:C2263130 | lld:lifeskim |
| pubmed-article:1650372 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:1650372 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:1650372 | pubmed:dateCreated | 1991-9-5 | lld:pubmed |
| pubmed-article:1650372 | pubmed:abstractText | We have studied the mechanisms involved in calcium (Ca2+) transport through the basal plasma membranes (BPM) of the syncytiotrophoblast cells from full-term human placenta. These purified membranes were enriched 25-fold in Na+/K(+)-adenosine triphosphate (ATPase), 37-fold in [3H] dihydroalprenolol binding sites, and fivefold in alkaline phosphatase activity compared with the placenta homogenates. In the absence of ATP and Mg2+, a basal Ca2+ uptake was observed, which followed Michaelis-Menten kinetics, with a Km Ca2+ of 0.18 +/- 0.05 microM and Vmax of 0.93 +/- 0.11 nmol/mg/min. The addition of Mg2+ to the incubation medium significantly decreased this uptake in a concentration-dependent manner, with a maximal inhibition at 3 mM Mg2+ and above. The Lineweaver-Burk plots of Ca2+ uptake in the absence and in the presence of 1 mM Mg2+ suggest a noncompetitive type of inhibition. Preloading the BPM vesicles with 5 mM Mg2+ had no significant effect on Ca2+ uptake, eliminating the hypothesis of a Ca2+/Mg2+ exchange mechanism. This ATP-independent Ca2+ uptake was not sensitive to 10(-6) M nitrendipine nor to 10(-4) M verapamil. An ATP-dependent Ca2+ transport was also detected in these BPM, whose Km Ca2+ was 0.09 +/- 0.02 microM and Vmax 3.4 +/- 0.2 nmoles/mg/3 min. This Ca2+ transport requires Mg2+, the optimal concentration of Mg2+ being approximately 1 mM. Preincubation of the membrane with 10(-6) M calmodulin strongly enhanced the initial ATP-dependent Ca2+ uptake. Finally, no Na+/Ca2+ exchange process could be demonstrated. | lld:pubmed |
| pubmed-article:1650372 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1650372 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1650372 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1650372 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1650372 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1650372 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1650372 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1650372 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1650372 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1650372 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1650372 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1650372 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1650372 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1650372 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1650372 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:1650372 | pubmed:issn | 0021-9541 | lld:pubmed |
| pubmed-article:1650372 | pubmed:author | pubmed-author:LeclercMM | lld:pubmed |
| pubmed-article:1650372 | pubmed:author | pubmed-author:BrunetteM GMG | lld:pubmed |
| pubmed-article:1650372 | pubmed:author | pubmed-author:LafondJJ | lld:pubmed |
| pubmed-article:1650372 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1650372 | pubmed:volume | 148 | lld:pubmed |
| pubmed-article:1650372 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1650372 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1650372 | pubmed:pagination | 17-23 | lld:pubmed |
| pubmed-article:1650372 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
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| pubmed-article:1650372 | pubmed:meshHeading | pubmed-meshheading:1650372-... | lld:pubmed |
| pubmed-article:1650372 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1650372 | pubmed:articleTitle | Characterization of calcium transport by basal plasma membranes from human placental syncytiotrophoblast. | lld:pubmed |
| pubmed-article:1650372 | pubmed:affiliation | Maisonneuve-Rosemont Hospital, Montreal, Quebec, Canada. | lld:pubmed |
| pubmed-article:1650372 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1650372 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1650372 | lld:pubmed |
