Linked Life Data

16503640

Source:http://linkedlifedata.com/resource/pubmed/id/16503640

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2006-2-28
pubmed:abstractText
In eukaryotic cells, chromatin is classified into euchromatin, which is active in transcription, and heterochromatin that silences transcription. Histones in these two domains contain distinct modifications. Chromatin assembly factor 1 (CAF-1) is a highly conserved protein that functions in DNA replication, DNA repair, and heterochromatin silencing. CAF-1 binds histones H3 and H4 and deposits histones onto DNA to form nucleosomes. However, modifications on H3 and H4 associated with CAF-1 are not known. Here, we have purified a complex containing CAF-1 and H3 and H4 from yeast cells and determined the modifications present on these histones using linear ion trap FT-ICR mass spectrometry. H4 that copurified with CAF-1 was a mixture of isoforms acetylated at lysines 5, 8, 12, and 16, whereas an H3 peptide methylated at lysine 79 and an H3 peptide acetylated at lysine 56 were detected. In yeast cell extracts, these two H3 modifications peaked in the late S phase with different kinetics. Moreover, the association of CAF-1 with H3 methylated at lysine 79 appeared to occur in the late S phase. Finally, cells lacking both Dot1p, the methyltransferase that methylates H3 lysine 79, and Cac1p, the large subunit of CAF-1, exhibited a dramatic loss of telomeric silencing and increased sensitivity to DNA damaging agents. Together, these data indicate that CAF-1 interacts with H3 methylated at lysine 79 during the processes of epigenetic silencing and DNA repair.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
7
pubmed:volume
45
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2852-61
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:16503640-Amino Acid Sequence, pubmed-meshheading:16503640-Cell Extracts, pubmed-meshheading:16503640-Chromatin Assembly Factor-1, pubmed-meshheading:16503640-Chromosomal Proteins, Non-Histone, pubmed-meshheading:16503640-DNA Repair, pubmed-meshheading:16503640-DNA-Binding Proteins, pubmed-meshheading:16503640-Epigenesis, Genetic, pubmed-meshheading:16503640-Gene Silencing, pubmed-meshheading:16503640-Histones, pubmed-meshheading:16503640-Lysine, pubmed-meshheading:16503640-Mass Spectrometry, pubmed-meshheading:16503640-Methylation, pubmed-meshheading:16503640-Molecular Sequence Data, pubmed-meshheading:16503640-Nucleosomes, pubmed-meshheading:16503640-S Phase, pubmed-meshheading:16503640-Saccharomyces cerevisiae, pubmed-meshheading:16503640-Silent Information Regulator Proteins, Saccharomyces...
pubmed:year
2006
pubmed:articleTitle
Chromatin assembly factor 1 interacts with histone H3 methylated at lysine 79 in the processes of epigenetic silencing and DNA repair.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, Minnesota 55905, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural