Linked Life Data

1650274

Source:http://linkedlifedata.com/resource/pubmed/id/1650274

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1991-8-30
pubmed:abstractText
Angiotensin-converting enzyme (ACE, kininase II, EC 3.4.15.1) was quantified in selected areas of the rat brain by 3 different methods. The enzyme activity was quantified by an enzymatic assay in homogenates of discrete brain areas with the use of the artificial substrate hippuryl-His-Leu-[14C]glycine. Binding of the specific ACE inhibitor [125I]351A was quantified after incubation of thin brain sections followed by quantitative autoradiography with comparison to [125I]standards. The antigenic sites of the enzyme were quantified by immunoautoradiography, using a polyclonal antibody to ACE in combination with [125I]protein A followed by quantitative autoradiography. There was a good correlation between the ACE activity, as determined by the quantitative enzymatic method, and both the enzyme inhibitor binding and the [125I]protein A binding, as determined by quantitative autoradiography, in brain areas and in anterior and posterior lobes of the pituitary gland. The present data demonstrate that ACE activity and ACE binding can be quantified in discrete tissue areas with different methods in a variety of conditions and with a high degree of accuracy.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-8993
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
545
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
215-22
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Comparative quantification of rat brain and pituitary angiotensin-converting enzyme with autoradiographic and enzymatic methods.
pubmed:affiliation
Section on Pharmacology, National Institute of Mental Health, Bethesda, MD 20892.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't