rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2006-5-22
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pubmed:abstractText |
Nucleic acids can quench resonance light scattering (RLS) intensity of the Y(III)-1,6-bi(1'-phenyl-3'-methyl-5'-pyrazolone-4'-)hexane-dione(BPMPHD) complex in the pH range 5.0-5.8. Under optimal conditions, there are linear relationships between the quenching of RLS and the concentration of nucleic acids in the range 6.3 x 10(-8)-2.1 x 10(-5) g/mL for fish sperm DNA (fsDNA), 1.2 x 10(-8)-5.0 x 10(-5) g/mL for calf thymus DNA (ctDNA) and 6.0 x 10(-8)-2.0 x 10(-5) g/mL for yeast RNA (yRNA). The detection limits (3 s) of fsDNA, ctDNA and yRNA are 0.7 ng/mL, 3.8 ng/mL and 4.2 ng/mL, respectively.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1522-7235
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pubmed:author |
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pubmed:copyrightInfo |
Copyright (c) 2006 John Wiley & Sons, Ltd.
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pubmed:issnType |
Print
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pubmed:volume |
21
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
129-34
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16502393-Aldehydes,
pubmed-meshheading:16502393-Animals,
pubmed-meshheading:16502393-Cattle,
pubmed-meshheading:16502393-DNA,
pubmed-meshheading:16502393-Fishes,
pubmed-meshheading:16502393-Hydrogen-Ion Concentration,
pubmed-meshheading:16502393-Male,
pubmed-meshheading:16502393-Nucleic Acids,
pubmed-meshheading:16502393-Pyrazoles,
pubmed-meshheading:16502393-RNA,
pubmed-meshheading:16502393-Scattering, Radiation,
pubmed-meshheading:16502393-Sensitivity and Specificity,
pubmed-meshheading:16502393-Spermatozoa,
pubmed-meshheading:16502393-Thymus Gland,
pubmed-meshheading:16502393-Yeasts
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pubmed:articleTitle |
Determination of nucleic acids based on the quenching effect on resonance light scattering of the Y(III)-1,6-bi(1'-phenyl-3'-methyl-5'-pyrazolone-4'-)hexane-dione system.
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pubmed:affiliation |
Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, People's Republic of China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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