Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2006-2-27
pubmed:abstractText
APOBEC3G (A3G) and related cytidine deaminases, such as APOBEC3F (A3F), are potent inhibitors of retroviruses. Formation of infectious human immunodeficiency virus type 1 (HIV-1) requires suppression of multiple cytidine deaminases by Vif. Whether HIV-1 Vif recognizes various APOBEC3 proteins through a common mechanism is unclear. The domains in Vif that mediate APOBEC3 recognitions are also poorly defined. The N-terminal region of HIV-1 Vif is unusually rich in Trp residues, which are highly conserved. In the present study, we examined the role of these Trp residues in the suppression of APOBEC3 proteins by HIV-1 Vif. We found that most of the highly conserved Trp residues were required for efficient suppression of both A3G and A3F, but some of these residues were selectively required for the suppression of A3F but not A3G. Mutant Vif molecules in which Ala was substituted for Trp79 and, to a lesser extent, for Trp11 remained competent for A3G interaction and its suppression; however, they were defective for A3F interaction and therefore could not efficiently suppress the antiviral activity of A3F. Interestingly, while the HIV-1 Vif-mediated degradation of A3G was not affected by the different C-terminal tag peptides, that of A3F was significantly influenced by its C-terminal tags. These data indicate that the mechanisms by which HIV-1 Vif recognizes its target molecules, A3G and A3F, are not identical. The fact that several highly conserved residues in Vif are required for the suppression of A3F but not that of A3G suggests a critical role for A3F in the restriction of HIV-1 in vivo.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-12167863, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-12750511, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-12808465, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-12808466, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-12809610, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-12859895, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-14527406, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-14528300, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-14528301, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-14564014, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-14614829, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-14747572, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-14966139, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-14978281, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-14999100, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-15054139, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-15098018, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-15121899, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-15141007, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-15152192, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-15177194, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-15245742, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-15296757, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-15296758, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-15353783, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-15466872, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-15496950, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-15516966, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-15574592, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-15574593, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-15610729, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-15674295, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-15718460, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-15726649, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-15781449, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-15809227, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-15823539, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-15911774, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-15919829, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-15993456, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-16000409, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-16014920, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-16076960, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-16201018, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-3006243, http://linkedlifedata.com/resource/pubmed/commentcorrection/16501124-9201229
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
80
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3112-5
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2006
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