Source:http://linkedlifedata.com/resource/pubmed/id/16497529
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2006-5-16
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pubmed:abstractText |
Metabolic and cognitive alterations occur during hyperammonemia. Here, we report that chronic hyperammonemia also leads to increased sensitivity to LPS. Sparse-fur mice were challenged i.p. with LPS or saline control and then tested for motivation to investigate a novel juvenile over 24 h. Cytokine, ammonia, and urea concentration were quantified at the peak of sickness (2 h post injection). Chronic hyperammonemic Otc(spf-ash) mice displayed more pronounced and prolonged sickness behavior in response to LPS (P=0.02). LPS significantly (P<0.0001) increased plasma concentrations of TNFalpha, IL-1 beta, IL-6, IL-15, IL-9, IL-2, IL-1 alpha, IL-1 beta, Rantes, MIP1 alpha, MIP1 beta, MCP-1, KC, GM-CSF, G-CSF, Eotaxin, IL-13, and IL-12 in both wild type and Otc(spf-ash) mice. No significant genotype/treatment interactions (P>0.1) were detected for any cytokine. Adult Otc(spf-ash) mice (168+/-41 microM) had four times higher plasma ammonia compared to wild type mice (40 +/- 6 microM) (P=0.002). Two hours after LPS injection, plasma ammonia concentrations tended (P=0.08) to decrease in both wild type and Otc(spf-ash) mice. Learning and memory behaviors were assessed in mice under basal conditions to determine the impact of chronic hyperammonemia on cognition. Otc(spf-ash) mice performed significantly poorer in the two trial Y-maze (P=0.02) and the Morris water maze (P=0.001) than their littermate wild type controls. Taken together, these data indicate that chronic hyperammonemia results in impaired cognition and creates a state of LPS hypersensitivity.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1096-7192
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
88
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
131-7
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:16497529-Animals,
pubmed-meshheading:16497529-Attention,
pubmed-meshheading:16497529-Cytokines,
pubmed-meshheading:16497529-Disease Models, Animal,
pubmed-meshheading:16497529-Hyperammonemia,
pubmed-meshheading:16497529-Inflammation,
pubmed-meshheading:16497529-Lipopolysaccharides,
pubmed-meshheading:16497529-Male,
pubmed-meshheading:16497529-Maze Learning,
pubmed-meshheading:16497529-Memory,
pubmed-meshheading:16497529-Mice,
pubmed-meshheading:16497529-Mice, Mutant Strains,
pubmed-meshheading:16497529-Ornithine Carbamoyltransferase Deficiency Disease,
pubmed-meshheading:16497529-Social Behavior,
pubmed-meshheading:16497529-Specific Pathogen-Free Organisms
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pubmed:year |
2006
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pubmed:articleTitle |
Hyperammonemia increases sensitivity to LPS.
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pubmed:affiliation |
Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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