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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2006-2-24
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pubmed:abstractText |
A major challenge in systems neuroscience is to perform precise molecular genetic analyses of a single neuronal population in the context of the complex mammalian brain. Existing technologies for profiling cell type-specific gene expression are largely limited to immature or morphologically identifiable neurons. In this study, we developed a simple method using fluorescent activated cell sorting (FACS) to purify genetically labeled neurons from juvenile and adult mouse brains for gene expression profiling. We identify and verify a new set of differentially expressed genes in the striatonigral and striatopallidal neurons, two functionally and clinically important projection neuron subtypes in the basal ganglia. We further demonstrate that Ebf1 is a lineage-specific transcription factor essential to the differentiation of striatonigral neurons. Our study provides a general approach for profiling cell type-specific gene expression in the mature mammalian brain and identifies a set of genes critical to the function and dysfunction of the striatal projection neuron circuit.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1097-6256
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
443-52
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:16491081-Aging,
pubmed-meshheading:16491081-Animals,
pubmed-meshheading:16491081-Cell Differentiation,
pubmed-meshheading:16491081-Cell Lineage,
pubmed-meshheading:16491081-DNA-Binding Proteins,
pubmed-meshheading:16491081-Flow Cytometry,
pubmed-meshheading:16491081-Gene Expression Profiling,
pubmed-meshheading:16491081-Gene Expression Regulation, Developmental,
pubmed-meshheading:16491081-Genetic Markers,
pubmed-meshheading:16491081-Globus Pallidus,
pubmed-meshheading:16491081-Mice,
pubmed-meshheading:16491081-Mice, Inbred C57BL,
pubmed-meshheading:16491081-Mice, Transgenic,
pubmed-meshheading:16491081-Neostriatum,
pubmed-meshheading:16491081-Neural Pathways,
pubmed-meshheading:16491081-Neurons,
pubmed-meshheading:16491081-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:16491081-RNA, Messenger,
pubmed-meshheading:16491081-Substantia Nigra,
pubmed-meshheading:16491081-Trans-Activators
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pubmed:year |
2006
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pubmed:articleTitle |
FACS-array profiling of striatal projection neuron subtypes in juvenile and adult mouse brains.
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pubmed:affiliation |
Center for Neurobehavioral Genetics, Semel Institute for Neuroscience and Human Behavior, Department of Psychiatry and Biobehavioral Sciences, University of California at Los Angeles, 90095, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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