Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1991-8-15
pubmed:abstractText
Gossypol, an antifertility agent, has inhibitory actions on many membrane-associated enzymes, suggesting that this agent might have a generalized effect on cell membranes. This hypothesis was examined in the present study using membranes and dispersed cells prepared from human and rat adrenal glands. Four parameters were determined: microviscosity as measured by fluorescence polarization of human adrenal microsomal- and mitochondrial-enriched membranes, adrenal steroidogenic enzymes; and cAMP and cortisol responses to ACTH. It was found that gossypol increased the polarization constants of microsomes and mitochondria in a dose-dependent manner. Of the three adrenal enzymes tested, both 3 beta-hydroxysteroid dehydrogenase delta 5-delta 4 isomerase and 11-hydroxylase were inhibited by gossypol, but not 21-hydroxylase. Using intact human adrenocortical cells, high doses of gossypol also inhibited the ACTH-stimulated cAMP and cortisol levels. The in vivo corticosterone response to ACTH in rats subjected to chronic gossypol treatment was also found to be reduced. These findings suggest that gossypol has multiple effects on adrenal function. Its effects on membrane microviscosity, adrenal steroidogenesis, cAMP and corticosterone responses to ACTH stimulation probably occur through a generalized membrane effect.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0001-5598
pubmed:author
pubmed:issnType
Print
pubmed:volume
124
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
672-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:1648853-Adrenal Glands, pubmed-meshheading:1648853-Adrenocorticotropic Hormone, pubmed-meshheading:1648853-Adult, pubmed-meshheading:1648853-Animals, pubmed-meshheading:1648853-Cells, Cultured, pubmed-meshheading:1648853-Corticosterone, pubmed-meshheading:1648853-Cyclic AMP, pubmed-meshheading:1648853-Female, pubmed-meshheading:1648853-Fluorescence Polarization, pubmed-meshheading:1648853-Gossypol, pubmed-meshheading:1648853-Humans, pubmed-meshheading:1648853-Hydrocortisone, pubmed-meshheading:1648853-Intracellular Membranes, pubmed-meshheading:1648853-Male, pubmed-meshheading:1648853-Microsomes, pubmed-meshheading:1648853-Mitochondria, pubmed-meshheading:1648853-Multienzyme Complexes, pubmed-meshheading:1648853-Progesterone Reductase, pubmed-meshheading:1648853-Rats, pubmed-meshheading:1648853-Rats, Inbred Strains, pubmed-meshheading:1648853-Steroid 11-beta-Hydroxylase, pubmed-meshheading:1648853-Steroid Isomerases, pubmed-meshheading:1648853-Viscosity
pubmed:year
1991
pubmed:articleTitle
Inhibitory effects of gossypol on adrenal function.
pubmed:affiliation
Developmental Endocrinology Branch, NICHD, NIH, Bethesda, Maryland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't