Linked Life Data

16487614

Source:http://linkedlifedata.com/resource/pubmed/id/16487614

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2006-3-28
pubmed:abstractText
This study aimed to investigate the benefits of combining the use of PEG-modified cationic gold nanoparticles with electroporation for in vivo gene delivery. PEG-modified cationic gold nanoparticles were prepared by NaBH(4) reduction of HAuCl(4) in the presence of 2-aminoethanethiol and mPEG-SH. Zeta-potential of the particles was nearly neutral (+0.1 mV). After forming complexes with plasmid DNA at a w/w ratio of 8.4, nanoparticle complexes were 90 nm for at least 60 min and showed a negative zeta-potential. After intravenous injection of DNA-nanoparticle complexes, 20% of gold were detected in blood at 120 min after injection and 5% of DNA were observed in blood after 5 min, suggesting that PEG-modified nanoparticles were stably circulating in the blood flow, but some of the DNA bound to particles degraded during circulation. When electroporation was applied to a lobe of the liver following injection of DNA-nanoparticle complexes, significant gene expression was specifically observed in the pulsed lobe. We concluded that PEG-modified nanoparticles maintained DNA more stably in the blood flow than in the case of naked DNA and electroporation assisted in restricted gene expression of circulating DNA in limited areas of the liver.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0168-3659
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
111
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
382-9
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Stabilizing of plasmid DNA in vivo by PEG-modified cationic gold nanoparticles and the gene expression assisted with electrical pulses.
pubmed:affiliation
Department of Applied Chemistry, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't