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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6331
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pubmed:dateCreated |
1991-8-15
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pubmed:abstractText |
In many cell types, receptor activation of phosphoinositidase C results in an initial release of intracellular Ca2+ stores followed by sustained Ca2+ entry across the plasma membrane. Inositol 1,4,5-trisphosphate is the mediator of the initial Ca2+ release, although its role in the mechanism underlying Ca2+ entry remains controversial. We have now used two techniques to introduce inositol phosphates into mouse lacrimal acinar cells and measure their effects on Ca2+ entry: microinjection into cells loaded with Fura-2, a fluorescent dye which allows the measurement of intracellular free calcium concentration by microspectrofluorimetry, and perfusion of patch clamp pipettes in the whole-cell configuration while monitoring the activity of Ca(2+)-activated K+ channels as an indicator of intracellular Ca2+. We report here that inositol 1,4,5-trisphosphate serves as a signal that is both necessary and sufficient for receptor activation of Ca2+ entry across the plasma membrane in these cells.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol 1,4,5-Trisphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol 1,4,5-Trisphosphate...,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Methacholine Chloride,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytoplasmic and Nuclear,
http://linkedlifedata.com/resource/pubmed/chemical/Terpenes,
http://linkedlifedata.com/resource/pubmed/chemical/Thapsigargin,
http://linkedlifedata.com/resource/pubmed/chemical/inositol 2,4,5-trisphosphate
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0028-0836
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
352
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
162-5
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pubmed:dateRevised |
2007-7-18
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pubmed:meshHeading |
pubmed-meshheading:1648669-Animals,
pubmed-meshheading:1648669-Calcium,
pubmed-meshheading:1648669-Calcium Channels,
pubmed-meshheading:1648669-Inositol 1,4,5-Trisphosphate,
pubmed-meshheading:1648669-Inositol 1,4,5-Trisphosphate Receptors,
pubmed-meshheading:1648669-Inositol Phosphates,
pubmed-meshheading:1648669-Lacrimal Apparatus,
pubmed-meshheading:1648669-Methacholine Chloride,
pubmed-meshheading:1648669-Mice,
pubmed-meshheading:1648669-Phosphorylation,
pubmed-meshheading:1648669-Potassium Channels,
pubmed-meshheading:1648669-Receptors, Cell Surface,
pubmed-meshheading:1648669-Receptors, Cytoplasmic and Nuclear,
pubmed-meshheading:1648669-Terpenes,
pubmed-meshheading:1648669-Thapsigargin
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pubmed:year |
1991
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pubmed:articleTitle |
Activation of Ca2+ entry into acinar cells by a non-phosphorylatable inositol trisphosphate.
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pubmed:affiliation |
Calcium Regulation Section, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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