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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2006-5-3
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pubmed:abstractText |
The Ca2+-activated pathways in Saccharomyces cerevisiae induce a delay in the onset of mitosis through the activation of Swe1p, a negative regulatory kinase that inhibits the Cdc28p/Clb complex. We isolated the YAP1 gene as a multicopy suppressor of calcium sensitivity owing to the loss of ZDS1, a negative regulator of SWE1 and CLN2 gene expression. YAP1 deletion on a zds1delta background exacerbated the Ca2+-related phenotype. Yap1p was degraded in a calcineurin-dependent manner when cells were exposed to calcium. In yap1delta cells, the expression level of the RPN4 gene encoding a transcription factor for the subunits of the ubiquitin-proteasome system was diminished. The deletion of YAP1 gene or RPN4 gene led to the accumulation of Swe1p and Cln2p. Yap1p was a substrate of calcineurin in vivo and in vitro. The calcineurin-mediated Yap1p degradation seems to be a long adaptive response that assures a G2 delay in response to a stress that causes the activation of the calcium signalling pathways.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16485023-10842755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16485023-11230131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16485023-11432834,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16485023-11700296,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16485023-11918814,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16485023-15073333,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16485023-15215673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16485023-2542125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16485023-7615550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16485023-7915005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16485023-8075109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16485023-8107671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16485023-8182076,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16485023-8253069,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16485023-8313910,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16485023-8647431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16485023-8816438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16485023-8816439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16485023-9130715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16485023-9521328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16485023-9716410
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CLN2 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Calcineurin,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/SWE1 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/YAP1 protein, S cerevisiae
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1469-221X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
519-24
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:16485023-Calcineurin,
pubmed-meshheading:16485023-Calcium,
pubmed-meshheading:16485023-Cell Cycle Proteins,
pubmed-meshheading:16485023-Cyclins,
pubmed-meshheading:16485023-G2 Phase,
pubmed-meshheading:16485023-Protein-Tyrosine Kinases,
pubmed-meshheading:16485023-Saccharomyces cerevisiae,
pubmed-meshheading:16485023-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:16485023-Stress, Physiological,
pubmed-meshheading:16485023-Transcription Factors,
pubmed-meshheading:16485023-Up-Regulation
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pubmed:year |
2006
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pubmed:articleTitle |
Involvement of calcineurin-dependent degradation of Yap1p in Ca2+-induced G2 cell-cycle regulation in Saccharomyces cerevisiae.
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pubmed:affiliation |
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima 739-8530, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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