Linked Life Data

16481595

Source:http://linkedlifedata.com/resource/pubmed/id/16481595

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2006-5-22
pubmed:abstractText
The bovine dominant follicle (DF) model was used to identify molecular mechanisms potentially involved in initial growth of DF during the low FSH milieu of ovarian follicular waves. Follicular fluid and RNA from granulosa and theca cells were harvested from 10 individual DF obtained between 2 and 5.5 days after emergence of the first follicular wave of the estrous cycle. Follicular fluid was subjected to RIA to determine estradiol (E) and progesterone (P) concentrations and RNA to cDNA microarray analysis and (or) quantitative real-time PCR. Results showed that DF growth was associated with a decrease in intrafollicular E:P ratio and in mRNA for the FSH receptor, estrogen receptor 2 (ER beta), inhibin alpha, activin A receptor type I, and a proliferation (cyclin D2) and two proapoptotic factors (apoptosis regulatory protein Siva, Fas [TNFRSF6]-associated via death domain) in granulosa cells. In contrast, mRNAs for the LH receptor in granulosa cells and for two antiapoptotic factors (TGFB1-induced antiapoptotic factor 1, LAG1 longevity assurance homolog 4 [Saccharomyces cerevisiae]) and one proapoptotic factor (tumor necrosis factor [ligand] superfamily, member 8) were increased in theca cells. We conclude that the bovine DF provides a unique model to identify novel genes potentially involved in survival and apoptosis of follicular cells and, importantly, to determine the FSH-, estradiol-, and LH-target genes regulating its growth and function. Results provide new molecular evidence for the hypothesis that DF experience a reduction in FSH dependence but acquire increased LH dependence as they grow during the low FSH milieu of follicular waves.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0006-3363
pubmed:author
pubmed:issnType
Print
pubmed:volume
74
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1051-9
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:16481595-Activin Receptors, Type I, pubmed-meshheading:16481595-Animals, pubmed-meshheading:16481595-Apoptosis, pubmed-meshheading:16481595-Apoptosis Regulatory Proteins, pubmed-meshheading:16481595-Cattle, pubmed-meshheading:16481595-Cell Survival, pubmed-meshheading:16481595-Estradiol, pubmed-meshheading:16481595-Estrogen Receptor beta, pubmed-meshheading:16481595-Female, pubmed-meshheading:16481595-Follicle Stimulating Hormone, pubmed-meshheading:16481595-Follicular Fluid, pubmed-meshheading:16481595-Gene Expression Regulation, pubmed-meshheading:16481595-Granulosa Cells, pubmed-meshheading:16481595-Luteinizing Hormone, pubmed-meshheading:16481595-Ovarian Follicle, pubmed-meshheading:16481595-Progesterone, pubmed-meshheading:16481595-RNA, Messenger, pubmed-meshheading:16481595-Receptors, FSH, pubmed-meshheading:16481595-Receptors, LH, pubmed-meshheading:16481595-Signal Transduction, pubmed-meshheading:16481595-Theca Cells
pubmed:year
2006
pubmed:articleTitle
Molecular evidence that growth of dominant follicles involves a reduction in follicle-stimulating hormone dependence and an increase in luteinizing hormone dependence in cattle.
pubmed:affiliation
Division of Cell Sciences, Faculty of Veterinary Medicine, University of Glasgow, Glasgow G61 1QH, United Kingdom. m.mihm@vet.gla.ac.uk
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't