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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2006-2-15
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pubmed:abstractText |
The purpose of this study was to demonstrate the cellular localization of cyclooxygenase-2 (COX-2) and caveolin-3 (Cav-3) in primarily cultured rat chondrocytes. In normal rat chondrocytes, we observed relatively high levels of Cav-3 and a very low level of COX-2 mRNA and protein. Upon treating the chondrocytes with 5 microM of CdCl(2) (Cd) for 6 hr, the expressions of COX-2 mRNA and protein were increased with the decreased Cav-3 mRNA and protein expressions. The detergent insoluble caveolae-rich membranous fractions that were isolated from the rat chondrocytes and treated with Cd contained the both proteins of both COX-2 and Cav-3 in a same fraction. The immuno-precipitation experiments showed complex formation between the COX-2 and Cav-3 in the rat chondrocytes. Purified COX-2 with glutathione S-transferase-fused COX-2 also showed complex formation with Cav-3. Confocal and electron microscopy also demonstrated the co-localization of COX-2 and Cav-3 in the plasma membrane. The results from our current study show that COX-2 and Cav-3 are co-localized in the caveolae of the plasma membrane, and they form a protein-protein complex. The co-localization of COX-2 with Cav-3 in the caveolae suggests that the caveolins might play an important role for regulating the function of COX-2.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16479074-10371510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16479074-10381057,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16479074-10461811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16479074-10966838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16479074-11432874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16479074-11675416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16479074-11824967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16479074-12124863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16479074-12189159,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16479074-12734696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16479074-15055526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16479074-15230350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16479074-15272234,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16479074-15768830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16479074-16039968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16479074-1833187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16479074-2271017,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16479074-8365485,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16479074-8663016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16479074-8783630,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16479074-8791446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16479074-8969167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16479074-9045678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16479074-9361015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16479074-9488658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16479074-9738464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16479074-9841091
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1011-8934
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
21
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
100-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:16479074-Animals,
pubmed-meshheading:16479074-Animals, Newborn,
pubmed-meshheading:16479074-Blotting, Western,
pubmed-meshheading:16479074-Cadmium Chloride,
pubmed-meshheading:16479074-Caveolae,
pubmed-meshheading:16479074-Caveolin 3,
pubmed-meshheading:16479074-Cell Membrane,
pubmed-meshheading:16479074-Cells, Cultured,
pubmed-meshheading:16479074-Chondrocytes,
pubmed-meshheading:16479074-Cyclooxygenase 2,
pubmed-meshheading:16479074-Gene Expression,
pubmed-meshheading:16479074-Immunoprecipitation,
pubmed-meshheading:16479074-Microscopy, Confocal,
pubmed-meshheading:16479074-Microscopy, Electron,
pubmed-meshheading:16479074-RNA, Messenger,
pubmed-meshheading:16479074-Rats,
pubmed-meshheading:16479074-Reverse Transcriptase Polymerase Chain Reaction
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pubmed:year |
2006
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pubmed:articleTitle |
Evidence for cyclooxygenase-2 association with caveolin-3 in primary cultured rat chondrocytes.
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pubmed:affiliation |
Department of Pharmacology and Toxicology, College of Medicine, Inha University, Incheon.
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pubmed:publicationType |
Journal Article
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