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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2006-2-17
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pubmed:databankReference |
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pubmed:abstractText |
The cyanobacterial metabolite apratoxin A (1) demonstrates potent cytotoxicity against tumor cell lines by a hitherto unknown mechanism. We have used functional genomics to elucidate the molecular basis for this activity. Gene expression profiling and DNA content analysis showed that apratoxin A induces G1-phase cell cycle arrest and apoptosis. Cell-based functional assays with a genome-wide collection of expression cDNAs showed that ectopic induction of fibroblast growth factor receptor (FGFR) signaling attenuates the apoptotic activity of apratoxin A. This natural product inhibited phosphorylation and activation of STAT3, a downstream effector of FGFR signaling. It also caused defects in FGF-dependent processes during zebrafish development, with concomitant reductions in expression levels of the FGF target gene mkp3. We conclude that apratoxin A mediates its antiproliferative activity through the induction of G1 cell cycle arrest and an apoptotic cascade, which is at least partially initiated through antagonism of FGF signaling via STAT3.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1552-4450
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
158-67
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pubmed:dateRevised |
2007-1-12
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pubmed:meshHeading |
pubmed-meshheading:16474387-Animals,
pubmed-meshheading:16474387-Apoptosis,
pubmed-meshheading:16474387-Cell Cycle,
pubmed-meshheading:16474387-Cell Proliferation,
pubmed-meshheading:16474387-Depsipeptides,
pubmed-meshheading:16474387-Drug Resistance, Neoplasm,
pubmed-meshheading:16474387-Endothelial Cells,
pubmed-meshheading:16474387-Fibroblast Growth Factors,
pubmed-meshheading:16474387-G1 Phase,
pubmed-meshheading:16474387-Gene Expression Regulation,
pubmed-meshheading:16474387-Genomics,
pubmed-meshheading:16474387-Humans,
pubmed-meshheading:16474387-Molecular Conformation,
pubmed-meshheading:16474387-Phosphorylation,
pubmed-meshheading:16474387-RNA, Messenger,
pubmed-meshheading:16474387-STAT3 Transcription Factor,
pubmed-meshheading:16474387-Signal Transduction,
pubmed-meshheading:16474387-Structure-Activity Relationship,
pubmed-meshheading:16474387-Transcription, Genetic,
pubmed-meshheading:16474387-Tumor Cells, Cultured,
pubmed-meshheading:16474387-Zebrafish
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pubmed:year |
2006
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pubmed:articleTitle |
A functional genomics approach to the mode of action of apratoxin A.
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pubmed:affiliation |
Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA. luesch@cop.ufl.edu
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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