Linked Life Data

16473580

Source:http://linkedlifedata.com/resource/pubmed/id/16473580

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2006-2-13
pubmed:abstractText
We present an in vitro method to measure how Rab4 and other regulatory proteins affect microtubule-based organelle motility. The protocols utilize small-volume, disposable "microchambers" designed for epifluorescence, confocal, or other microscope platforms and into which microtubules, organelles, and primary and fluorescent secondary antibodies are added. Our work has focused on the isolation and use of endocytic vesicles from rat liver, and we present these protocols. However, the techniques can be adapted for other organelles or cell types. Multiple fluorescent probes, rapid image capture, and immunofluorescence under non-fixation conditions allow for measurements of the location and intensity changes of endogenous proteins upon addition of ATP or upon addition of other proteins or regulatory factors. We review measurements of microtubule-based motility as well as measurements for protein localization and protein segregation in vitro.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0076-6879
pubmed:author
pubmed:issnType
Print
pubmed:volume
403
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
92-107
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Assay of Rab4-dependent trafficking on microtubules.
pubmed:publicationType
Journal Article