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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
16
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pubmed:dateCreated |
2006-3-27
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pubmed:abstractText |
Here we conduct dose-response studies for in vitro expression and in vivo immunogenicity for a recombinant modified vaccinia Ankara (MVA) vaccine that expresses HIV Gag, Pol, and Env proteins. The dose-response studies for in vitro expression used fluorescent-activated cell sorting to score Gag- and Env-expressing cells and showed good increases for antigen expression with increasing MVA dose. In these studies, a 1000-fold increase in the dose of MVA resulted in a 300-fold increase in the frequency of antigen-expressing cells. In contrast, dose-response studies for in vivo immunogenicity showed <10-fold increases in elicited T cells and Ab for 100-1000-fold increases in the dose of inoculated MVA. These studies used intracellular cytokine assays to enumerate responding CD8 and CD4 T cells and an ELISA to score anti-Env Ab. The shallow dose-response curves for immunogenicity were observed post priming as well as post boosting of an MVA or a DNA prime.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/AIDS Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, env,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, gag,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, pol,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, Synthetic,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Vaccines
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0264-410X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
12
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3332-9
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:16472543-AIDS Vaccines,
pubmed-meshheading:16472543-Animals,
pubmed-meshheading:16472543-CD4-Positive T-Lymphocytes,
pubmed-meshheading:16472543-CD8-Positive T-Lymphocytes,
pubmed-meshheading:16472543-Cell Line,
pubmed-meshheading:16472543-Cytokines,
pubmed-meshheading:16472543-Dose-Response Relationship, Immunologic,
pubmed-meshheading:16472543-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:16472543-Female,
pubmed-meshheading:16472543-Gene Expression,
pubmed-meshheading:16472543-Gene Products, env,
pubmed-meshheading:16472543-Gene Products, gag,
pubmed-meshheading:16472543-Gene Products, pol,
pubmed-meshheading:16472543-HIV Antibodies,
pubmed-meshheading:16472543-Humans,
pubmed-meshheading:16472543-Lymphocyte Subsets,
pubmed-meshheading:16472543-Mice,
pubmed-meshheading:16472543-Mice, Inbred BALB C,
pubmed-meshheading:16472543-Vaccines, DNA,
pubmed-meshheading:16472543-Vaccines, Synthetic,
pubmed-meshheading:16472543-Vaccinia virus,
pubmed-meshheading:16472543-Viral Vaccines
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pubmed:year |
2006
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pubmed:articleTitle |
Studies on in vitro expression and in vivo immunogenicity of a recombinant MVA HIV vaccine.
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pubmed:affiliation |
Emory Vaccine Center, Emory University School of Medicine and Yerkes National Primate Research Center, 954 Gatewood Road, Box 129, Atlanta, GA, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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