Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2006-6-12
pubmed:abstractText
Numerous studies have demonstrated the effect of shear stress on osteoblasts, but its effect on odontogenic cells has never been reported. In this study, we focused on the effect of shear stress on facilitating tissue-engineered odontogenesis by dissociated single cells. Cells were harvested from the porcine third molar tooth at the early stage of crown formation, and the isolated heterogeneous cells were seeded on a biodegradable polyglycolic acid fiber mesh. Then, cell-polymer constructs with and without exposure to shear stress were evaluated by in vitro and in vivo studies. In in vitro studies, the expression of both epithelial and mesenchymal odontogenic-related mRNAs was significantly enhanced by shear stress for 2 h. At 12 h after exposure to shear stress, the expression of amelogenin, bone sialoprotein and vimentin protein was significantly enhanced compared with that of control. Moreover, after 7 days, alkaline phosphatase activity exhibited a significant increase without any significant effect on cell proliferation in vitro. In vivo, enamel and dentin tissues formed after 15 weeks of in vivo implantation in constructs exposure to in vitro shear stress for 12 h. Such was not the case in controls. We concluded that shear stress facilitates odontogenic cell differentiation in vitro as well as the process of tooth tissue engineering in vivo.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
8756-3282
pubmed:author
pubmed:issnType
Print
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
125-33
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:16469551-Alkaline Phosphatase, pubmed-meshheading:16469551-Amelogenin, pubmed-meshheading:16469551-Animals, pubmed-meshheading:16469551-Biocompatible Materials, pubmed-meshheading:16469551-Biodegradation, Environmental, pubmed-meshheading:16469551-Cell Culture Techniques, pubmed-meshheading:16469551-Cell Differentiation, pubmed-meshheading:16469551-Cells, Cultured, pubmed-meshheading:16469551-Dental Enamel, pubmed-meshheading:16469551-Dental Enamel Proteins, pubmed-meshheading:16469551-Dentin, pubmed-meshheading:16469551-Epithelium, pubmed-meshheading:16469551-Integrin-Binding Sialoprotein, pubmed-meshheading:16469551-Mesoderm, pubmed-meshheading:16469551-Molar, Third, pubmed-meshheading:16469551-Odontogenesis, pubmed-meshheading:16469551-Polyglycolic Acid, pubmed-meshheading:16469551-Polymers, pubmed-meshheading:16469551-RNA, Messenger, pubmed-meshheading:16469551-Sialoglycoproteins, pubmed-meshheading:16469551-Stress, Mechanical, pubmed-meshheading:16469551-Swine, pubmed-meshheading:16469551-Time Factors, pubmed-meshheading:16469551-Tissue Engineering, pubmed-meshheading:16469551-Tooth Germ, pubmed-meshheading:16469551-Vimentin
pubmed:year
2006
pubmed:articleTitle
Shear stress facilitates tissue-engineered odontogenesis.
pubmed:affiliation
Tooth Regeneration, The Division of Stem Cell Engineering, The Institute of Medical Science, The University of Tokyo, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan. honda-m@ims.u-tokyo.ac.jp
pubmed:publicationType
Journal Article, Comparative Study, In Vitro, Research Support, Non-U.S. Gov't