Linked Life Data

16469199

Source:http://linkedlifedata.com/resource/pubmed/id/16469199

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 6
pubmed:dateCreated
2006-5-16
pubmed:abstractText
The microsporidian Encephalitozoon cuniculi is an obligate intracellular parasite that develops asynchronously inside parasitophorous vacuoles. Spore differentiation involves the construction of a cell wall commonly divided into an outer layer (exospore) and a thicker, chitin-rich inner layer (endospore). The developmental patterns of protein deposition and mRNA expression for 2 different spore wall proteins were studied using immunocytochemical and in situ hybridization procedures with ultrathin frozen sections. The onset of deposition of an exospore-destined protein (SWP1) correlated with the formation of lamellar protuberances during meront-to-sporont conversion. No evidence for a release of SWP1 towards the parasitophorous vacuole lumen was obtained. An endospore-destined protein (EnP1) was detected early on the plasma membrane of meronts prior to extensive accumulation within the chitin-rich layer of sporoblasts. swp1 mRNA was preferentially synthesized in early sporogony while enp1 mRNA was transcribed during merogony and a large part of sporogony. The level of both mRNAs was reduced in mature spores. Considering the availability of the E. cuniculi genome sequence, the application of nucleic and/or protein probes to cryosections should facilitate the screening of various genes for stage-specific expression during microsporidian development.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0031-1820
pubmed:author
pubmed:issnType
Print
pubmed:volume
132
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
815-25
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:16469199-Animals, pubmed-meshheading:16469199-Antibodies, Fungal, pubmed-meshheading:16469199-Cell Membrane, pubmed-meshheading:16469199-Cell Wall, pubmed-meshheading:16469199-Cells, Cultured, pubmed-meshheading:16469199-DNA Primers, pubmed-meshheading:16469199-Encephalitozoon cuniculi, pubmed-meshheading:16469199-Frozen Sections, pubmed-meshheading:16469199-Fungal Proteins, pubmed-meshheading:16469199-Gene Expression Regulation, Developmental, pubmed-meshheading:16469199-Gold, pubmed-meshheading:16469199-Immunohistochemistry, pubmed-meshheading:16469199-In Situ Hybridization, pubmed-meshheading:16469199-Life Cycle Stages, pubmed-meshheading:16469199-Microscopy, Electron, Transmission, pubmed-meshheading:16469199-RNA, Messenger, pubmed-meshheading:16469199-Spores, Fungal
pubmed:year
2006
pubmed:articleTitle
Expression of two cell wall proteins during the intracellular development of Encephalitozoon cuniculi: an immunocytochemical and in situ hybridization study with ultrathin frozen sections.
pubmed:affiliation
Equipe Parasitologie Moléculaire et Cellulaire, LBP, UMR CNRS 6023, Université Blaise Pascal, 24 Avenue des Landais, 63177 Aubière Cedex, France.
pubmed:publicationType
Journal Article, Comparative Study