Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1991-7-23
|
| pubmed:abstractText |
A combined diagnostic system for human papilloma virus (HPV) infections comprising the Papanicolaou test and in-situ hybridization assay was evaluated. Cervical smears from 259 women obtained with a "Cytobrush" were screened. Human papilloma virus genotypes 6/11, 16/18, 31/35/51 were detected by biotin in-situ hybridization in conjunction with a streptavidin-alkaline phosphatase detection complex. The diagnostic sensitivity of this assay was tested by human papilloma virus-DNA-positive human cervical carcinoma cell lines. According to the cytological (Bethesda system) and colposcopical criteria a random control group (n = 80) and prevention (n = 179) were chosen. Compared with Papanicolaou tests the frequency of human papilloma virus-DNA-positive cervices rose with the severity of cell abnormalities. The detection rate of human papilloma viruses-16/18 and human papilloma viruses-31/35/51 and of concomitant infections with human papilloma viruses-6/11 and human papilloma viruses-16/18 and/or human papilloma viruses-31/35/51 increased with the severity of cell dysplasia, whereas the rate of human papilloma virus-6/11 DNAs decreased. The incidence of oncogenic human papilloma virus types 16/18 and 31/35/51 rose with the age of the patients. A follow-up study by Papanicolaou tests of patients with mild (slight) and moderate dysplasias six months after human papilloma virus-DNA-hybridization indicates that human papilloma virus-16/18 DNA-positive lesions are more likely to persist or to progress than human papilloma virus-6/11 DNA-positive cell changes. Human papilloma virus-31/35/51 DNA-positive cell smears exhibited persistent behaviour. Our findings demonstrate that the Papanicolaou test combined with in-situ hybridization is suitable for early diagnosis and prevention of intraepithelial neoplasias and carcinomas of the uterine cervix.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0939-4974
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
29
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
131-8
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:1646654-Adolescent,
pubmed-meshheading:1646654-Adult,
pubmed-meshheading:1646654-Age Factors,
pubmed-meshheading:1646654-Aged,
pubmed-meshheading:1646654-Carcinoma, Squamous Cell,
pubmed-meshheading:1646654-Child,
pubmed-meshheading:1646654-Female,
pubmed-meshheading:1646654-Genotype,
pubmed-meshheading:1646654-Humans,
pubmed-meshheading:1646654-Middle Aged,
pubmed-meshheading:1646654-Nucleic Acid Hybridization,
pubmed-meshheading:1646654-Papillomaviridae,
pubmed-meshheading:1646654-Prognosis,
pubmed-meshheading:1646654-Tumor Virus Infections,
pubmed-meshheading:1646654-Uterine Cervical Dysplasia,
pubmed-meshheading:1646654-Uterine Cervical Neoplasms,
pubmed-meshheading:1646654-Vaginal Smears
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
Papanicolaou test and enzyme-linked in-situ hybridization. A combined diagnostic system for papilloma virus infections with high prognostic value.
|
| pubmed:affiliation |
Institut für Medizinische Chemie, Universität Wien.
|
| pubmed:publicationType |
Journal Article
|