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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1975-6-25
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pubmed:abstractText |
The template activities of the 60-70S RNA complex and of the 30-40S subunit RNA species of Rous sarcoma virus were tested in a cell-free protein-synthesizing system from mouse ascites Krebs II cells. Stimulation of protein synthesis over the endogenous background was about 2-fold with 30-40S viral RNA and about 1.3-fold with 60-70S viral RNA as template. Analysis by sodium dodecyl sulfate-gel electrophoresis showed that the predominant polypeptide synthesized in vitro in response to 30-40S RNA of Rous sarcoma virus had a molecular weight of 75,000-80,000. This polypeptide could be precipitated by antiserum against the group-specific antigens of the virus, although its molecular weight is higher than that of virion group-specific antigen proteins. Analysis of tryptic digests of the protein made in vitro indicates similarity to tryptic digests from authentic virion group-specific proteins. It is concluded that part of the RNA from Rous sarcoma virus is translated in vitro into a high-molecular-weight protein, perhaps a precursor of the virion group-specific proteins.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/164661-14174396,
http://linkedlifedata.com/resource/pubmed/commentcorrection/164661-4119788,
http://linkedlifedata.com/resource/pubmed/commentcorrection/164661-4317863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/164661-4321342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/164661-4321347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/164661-4332812,
http://linkedlifedata.com/resource/pubmed/commentcorrection/164661-4333742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/164661-4337245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/164661-4338254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/164661-4338637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/164661-4339194,
http://linkedlifedata.com/resource/pubmed/commentcorrection/164661-4346034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/164661-4351047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/164661-4352653,
http://linkedlifedata.com/resource/pubmed/commentcorrection/164661-4352969,
http://linkedlifedata.com/resource/pubmed/commentcorrection/164661-4357879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/164661-4370009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/164661-4372615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/164661-4372625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/164661-4524617,
http://linkedlifedata.com/resource/pubmed/commentcorrection/164661-4762415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/164661-4798989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/164661-5017913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/164661-5500401
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
72
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
614-8
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pubmed:dateRevised |
2010-9-1
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pubmed:meshHeading |
pubmed-meshheading:164661-Animals,
pubmed-meshheading:164661-Autoradiography,
pubmed-meshheading:164661-Avian Sarcoma Viruses,
pubmed-meshheading:164661-Carcinoma, Krebs 2,
pubmed-meshheading:164661-Cell-Free System,
pubmed-meshheading:164661-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:164661-Immunoassay,
pubmed-meshheading:164661-Methionine,
pubmed-meshheading:164661-Molecular Weight,
pubmed-meshheading:164661-Precipitin Tests,
pubmed-meshheading:164661-Protein Biosynthesis,
pubmed-meshheading:164661-RNA, Viral,
pubmed-meshheading:164661-Viral Proteins
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pubmed:year |
1975
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pubmed:articleTitle |
Translation of Rous sarcoma virus RNA in a cell-free system from ascites Krebs II cells.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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