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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2006-2-8
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pubmed:abstractText |
The formation of G-quadruplex and i-motif structures in the 5' end of the retinoblastoma (Rb) gene was examined using chemical modifications, circular dichroism (CD) and fluorescence spectroscopy. It was found that substitutions of 8-methylguanine at positions that show syn conformations in antiparallel G-quadruplexes stabilize the structure in the G-rich strand. The complementary C-rich 18mer forms an i-motif structure, as suggested by CD spectroscopy. Based on the C to T mutation experiments, C bases participated in the C-C+ base pair of the i-motif structure were determined. Experiments of 2-aminopurine (2-AP) substitution reveal that an increase of fluorescence in the G-quadruplex relative to duplex is attributed to unstacked 2-AP within the loop of G-quadruplex. The fluorescence experiments suggest that formation of the G-quadruplex and i-motif can compete with duplex formation. Furthermore, a polymerase arrest assay indicated that formation the G-quadruplex structure in the Rb gene acts as a barrier in DNA synthesis.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1362-4962
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
34
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
949-54
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:16464825-5' Flanking Region,
pubmed-meshheading:16464825-Circular Dichroism,
pubmed-meshheading:16464825-Cytosine,
pubmed-meshheading:16464825-DNA,
pubmed-meshheading:16464825-G-Quadruplexes,
pubmed-meshheading:16464825-Genes, Retinoblastoma,
pubmed-meshheading:16464825-Guanine,
pubmed-meshheading:16464825-Humans,
pubmed-meshheading:16464825-Spectrometry, Fluorescence
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pubmed:year |
2006
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pubmed:articleTitle |
Formation of the G-quadruplex and i-motif structures in retinoblastoma susceptibility genes (Rb).
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pubmed:affiliation |
Department of Chemistry, Graduate School of Science, Kyoto University Sakyo, Kyoto 606-8502, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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