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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2006-5-22
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pubmed:abstractText |
To facilitate a more efficient radiation and chemotherapy of mammary tumours, synthetic enhancer elements responsive to hypoxia and ionizing radiation were coupled to the mammary-specific minimal promoter of the murine whey acidic protein (WAP) encoding gene. The modified WAP promoter was introduced into a retroviral promoter conversion (ProCon) vector. Expression of a transduced reporter gene in response to hypoxia and radiation was analysed in stably infected mammary cancer cell lines and an up to 9-fold increase in gene expression demonstrated in comparison to the respective basic vector. Expression analyses in vitro, moreover, demonstrated a widely preserved mammary cell-specific promoter activity. For in vivo analyses, xenograft tumours consisting of infected human mammary adenocarcinoma cells were established in SCID/beige mice. Immunohistochemical analyses demonstrated a hypoxia-specific, markedly increased WAP promoter-driven expression in these tumours. Thus, this retroviral vector will facilitate a targeted gene therapeutic approach exploiting the unique environmental condition in solid tumours.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0042-6822
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
349
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
121-33
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:16464484-Animals,
pubmed-meshheading:16464484-Breast Neoplasms,
pubmed-meshheading:16464484-Cell Hypoxia,
pubmed-meshheading:16464484-Cell Line,
pubmed-meshheading:16464484-Disease Models, Animal,
pubmed-meshheading:16464484-Gene Expression Regulation,
pubmed-meshheading:16464484-Gene Therapy,
pubmed-meshheading:16464484-Genes, Reporter,
pubmed-meshheading:16464484-Genetic Vectors,
pubmed-meshheading:16464484-Green Fluorescent Proteins,
pubmed-meshheading:16464484-Humans,
pubmed-meshheading:16464484-Immunohistochemistry,
pubmed-meshheading:16464484-Mice,
pubmed-meshheading:16464484-Mice, SCID,
pubmed-meshheading:16464484-Microscopy, Fluorescence,
pubmed-meshheading:16464484-Milk Proteins,
pubmed-meshheading:16464484-Neoplasm Transplantation,
pubmed-meshheading:16464484-Promoter Regions, Genetic,
pubmed-meshheading:16464484-Retroviridae,
pubmed-meshheading:16464484-Sensitivity and Specificity,
pubmed-meshheading:16464484-Transplantation, Heterologous,
pubmed-meshheading:16464484-X-Rays
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pubmed:year |
2006
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pubmed:articleTitle |
Hypoxia- and radiation-inducible, breast cell-specific targeting of retroviral vectors.
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pubmed:affiliation |
Research Institute of Virology and Biomedicine, University of Veterinary Medicine, Veterinaerplatz 1, A-1210 Vienna, Austria.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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