Linked Life Data

16463256

Source:http://linkedlifedata.com/resource/pubmed/id/16463256

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2006-2-9
pubmed:abstractText
Atrazine is a widely used herbicide that was considered to be an endocrine disrupter capable of interfering with the synthesis and action of natural hormones. In the present study, we found that atrazine was able to cause apoptosis in grass carp (Ctenopharyngodon idellus) cells from cell line ZC7901. By fluorescent and transmission electron microscopy, the atrazine-incubated cells displayed a series of morphological changes, including condensation of the nucleus, margination of chromatin to form dense granular caps, and formation of apoptotic bodies. Moreover, DNA fragmentation was detected by the TUNEL reaction and agarose gel electrophoresis. These typical characteristics of cells undergoing apoptosis indicated the occurrence of apoptosis in ZC7901. Apoptosis induced by atrazine was dose- and time-dependent and was involved in mitochondrial membrane potential (DeltaPsi(m)) disruption, elevation in intracellular Ca(2+), generation of reactive oxygen species, and intracellular ATP depletion. This study provides the first evidence that atrazine was able to induce apoptosis in fish cells, which indicated the existence of a novel cytotoxic mechanism caused by atrazine and may improve our understanding of the complex relationship between contaminants and aquatic organisms.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1520-4081
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
80-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Cytotoxic effects and apoptosis induction of atrazine in a grass carp (Ctenopharyngodon idellus) cell line.
pubmed:affiliation
College of Life Sciences, Zhejiang University, Hangzhou 310012, People's Republic of China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't