|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2006-2-7
|
|
pubmed:abstractText |
A multiple vector system for the production and export of recombinant affinity-tagged proteins in Bacillus megaterium was developed. Up to 1 mg/liter of a His6-tagged or Strep-tagged Lactobacillus reuteri levansucrase was directed into the growth medium, using the B. megaterium esterase LipA signal peptide, and recovered by one-step affinity chromatography.
|
|
pubmed:commentsCorrections |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Feb
|
|
pubmed:issn |
0099-2240
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
72
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1677-9
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:16461726-Affinity Labels,
pubmed-meshheading:16461726-Bacillus megaterium,
pubmed-meshheading:16461726-Bacterial Proteins,
pubmed-meshheading:16461726-Chromatography, Affinity,
pubmed-meshheading:16461726-Culture Media,
pubmed-meshheading:16461726-Gene Expression,
pubmed-meshheading:16461726-Genes, Bacterial,
pubmed-meshheading:16461726-Genetic Vectors,
pubmed-meshheading:16461726-Hexosyltransferases,
pubmed-meshheading:16461726-Lactobacillus reuteri,
pubmed-meshheading:16461726-Plasmids,
pubmed-meshheading:16461726-Protein Sorting Signals,
pubmed-meshheading:16461726-Recombinant Fusion Proteins
|
|
pubmed:year |
2006
|
|
pubmed:articleTitle |
A Bacillus megaterium plasmid system for the production, export, and one-step purification of affinity-tagged heterologous levansucrase from growth medium.
|
|
pubmed:affiliation |
Institute of Microbiology, Technical University Braunschweig, Spielmannstrasse 7, D-38106 Braunschweig, Germany.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
