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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2006-2-14
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pubmed:abstractText |
By co-expression of heme oxygenase and various bilin reductase(s) in a single operon in conjunction with apophytochrome using two compatible plasmids, we developed a system to produce phytochromes with various chromophores in Escherichia coli. Through the selection of different bilin reductases, apophytochromes were assembled with phytochromobilin, phycocyanobilin, and phycoerythrobilin. The blue-shifted difference spectra of truncated phytochromes were observed with a phycocyanobilin chromophore compared to a phytochromobilin chromophore. When the phycoerythrobilin biosynthetic enzymes were co-expressed, E. coli cells accumulated orange-fluorescent phytochrome. The metabolic engineering of bacteria for the production of various bilins for assembly into phytochromes will facilitate the molecular analysis of photoreceptors.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bile Pigments,
http://linkedlifedata.com/resource/pubmed/chemical/Biliverdine,
http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Heme Oxygenase (Decyclizing),
http://linkedlifedata.com/resource/pubmed/chemical/Phycobilins,
http://linkedlifedata.com/resource/pubmed/chemical/Phycocyanin,
http://linkedlifedata.com/resource/pubmed/chemical/Phycoerythrin,
http://linkedlifedata.com/resource/pubmed/chemical/Phytochrome,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrapyrroles,
http://linkedlifedata.com/resource/pubmed/chemical/phycocyanobilin,
http://linkedlifedata.com/resource/pubmed/chemical/phycoerythrobilin,
http://linkedlifedata.com/resource/pubmed/chemical/phytochromobilin
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
580
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1333-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16458890-Bacteria,
pubmed-meshheading:16458890-Bile Pigments,
pubmed-meshheading:16458890-Biliverdine,
pubmed-meshheading:16458890-Escherichia coli,
pubmed-meshheading:16458890-Escherichia coli Proteins,
pubmed-meshheading:16458890-Fluorescent Dyes,
pubmed-meshheading:16458890-Heme Oxygenase (Decyclizing),
pubmed-meshheading:16458890-Phycobilins,
pubmed-meshheading:16458890-Phycocyanin,
pubmed-meshheading:16458890-Phycoerythrin,
pubmed-meshheading:16458890-Phytochrome,
pubmed-meshheading:16458890-Protein Engineering,
pubmed-meshheading:16458890-Tetrapyrroles
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pubmed:year |
2006
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pubmed:articleTitle |
Metabolic engineering to produce phytochromes with phytochromobilin, phycocyanobilin, or phycoerythrobilin chromophore in Escherichia coli.
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pubmed:affiliation |
Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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