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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
2006-9-13
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pubmed:abstractText |
Upregulation of specific transcription factors is a generally accepted mechanism to explain the commitment of hematopoietic stem cells along precise maturation lineages. Based on this premise, transduction of primary hematopoietic stem/progenitor cells with viral vectors containing the investigated transcription factors appears as a suitable experimental model to identify such regulators. Although MafB transcription factor is believed to play a role in the regulation of monocytic commitment, no demonstration is, to date, available supporting this function in normal human hematopoiesis. To address this issue, we retrovirally transduced cord blood CD34+ hematopoietic progenitors with a MafB cDNA. Immunophenotypic and morphological analysis of transduced cells demonstrated the induction of a remarkable monomacrophage differentiation. Microarray analysis confirmed these findings and disclosed the upregulation of macrophage-related transcription factors belonging to the AP-1, MAF, PPAR and MiT families. Altogether our data allow to conclude that MafB is a key regulator of human monocytopoiesis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1350-9047
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pubmed:author |
pubmed-author:FerrariSS,
pubmed-author:GemelliCC,
pubmed-author:GrandeAA,
pubmed-author:ManfrediniRR,
pubmed-author:MontanariMM,
pubmed-author:SalatiSS,
pubmed-author:SienaMM,
pubmed-author:TagliaficoEE,
pubmed-author:TenediniEE,
pubmed-author:VignudelliTT,
pubmed-author:Zanocco MaraniTT,
pubmed-author:ZinnWW
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pubmed:issnType |
Print
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pubmed:volume |
13
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1686-96
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16456583-Antigens, CD34,
pubmed-meshheading:16456583-Cell Line,
pubmed-meshheading:16456583-Colony-Forming Units Assay,
pubmed-meshheading:16456583-DNA, Complementary,
pubmed-meshheading:16456583-Fetal Blood,
pubmed-meshheading:16456583-Gene Expression Profiling,
pubmed-meshheading:16456583-Hematopoietic Stem Cells,
pubmed-meshheading:16456583-Humans,
pubmed-meshheading:16456583-Infant, Newborn,
pubmed-meshheading:16456583-MafB Transcription Factor,
pubmed-meshheading:16456583-Monocytes,
pubmed-meshheading:16456583-Myelopoiesis,
pubmed-meshheading:16456583-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:16456583-RNA, Small Interfering,
pubmed-meshheading:16456583-Retroviridae,
pubmed-meshheading:16456583-Transduction, Genetic,
pubmed-meshheading:16456583-Up-Regulation
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pubmed:year |
2006
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pubmed:articleTitle |
Virally mediated MafB transduction induces the monocyte commitment of human CD34+ hematopoietic stem/progenitor cells.
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pubmed:affiliation |
Department of Biomedical Sciences, University of Modena and Reggio Emilia, Modena, Italy.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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