Source:http://linkedlifedata.com/resource/pubmed/id/16456536
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2006-3-16
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pubmed:abstractText |
Intracellular IL-1 receptor antagonist (icIL-1ra) is reportedly involved in functions independent of blocking IL-1 receptor signaling. Fibroblasts derived from the involved skin of patients with systemic sclerosis (SSc) are predominantly of the myofibroblast phenotype, with higher levels of icIL-1ra compared to normal skin fibroblasts. We examined the effect of overexpression of icIL-1ra on the phenotype and function of normal fibroblasts with respect to the expression of alpha smooth muscle actin (alpha-SMA), a specific marker for myofibroblasts, and plasminogen activator inhibitor (PAI), a protein involved in fibrogenesis and expressed at higher levels in myofibroblasts, and the production of collagenase (matrix metalloproteinase-1 (MMP-1)), the major enzyme involved in the degradation of native collagen in the skin. Normal human foreskin fibroblasts overexpressing icIL-1ra showed higher levels of alpha-SMA and PAI and had lower levels of collagenase and MMP-1 mRNA induced by inflammatory cytokines. By contrast, levels of mRNA for tissue inhibitor of metalloproteinase-1 in the transfected cells were not different from the control cells. Pretreatment of the ic-IL-1ra-transfected cells with antisense oligonucleotide directed against the mRNA of icIL-1ra restored MMP-1 expression induced by stimulation with IL-1beta. Our data indicate novel functions for icIL-1ra, which might be relevant to the genesis of fibrotic diseases such as SSc.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Actins,
http://linkedlifedata.com/resource/pubmed/chemical/IL1RN protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin 1 Receptor Antagonist...,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/Plasminogen Inactivators,
http://linkedlifedata.com/resource/pubmed/chemical/Sialoglycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tissue Inhibitor of...,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-202X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
126
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
756-65
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:16456536-Actins,
pubmed-meshheading:16456536-Dermis,
pubmed-meshheading:16456536-Fibroblasts,
pubmed-meshheading:16456536-Fibrosis,
pubmed-meshheading:16456536-Humans,
pubmed-meshheading:16456536-Interleukin 1 Receptor Antagonist Protein,
pubmed-meshheading:16456536-Matrix Metalloproteinase 1,
pubmed-meshheading:16456536-Oligodeoxyribonucleotides, Antisense,
pubmed-meshheading:16456536-Phenotype,
pubmed-meshheading:16456536-Plasminogen Inactivators,
pubmed-meshheading:16456536-Scleroderma, Systemic,
pubmed-meshheading:16456536-Sialoglycoproteins,
pubmed-meshheading:16456536-Skin,
pubmed-meshheading:16456536-Tissue Inhibitor of Metalloproteinase-1,
pubmed-meshheading:16456536-Transcriptional Activation,
pubmed-meshheading:16456536-Tumor Necrosis Factor-alpha
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pubmed:year |
2006
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pubmed:articleTitle |
Novel functions of intracellular IL-1ra in human dermal fibroblasts: implications in the pathogenesis of fibrosis.
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pubmed:affiliation |
Department of Medicine, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, N.I.H., Extramural
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