Linked Life Data

1645031

Source:http://linkedlifedata.com/resource/pubmed/id/1645031

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1992-9-8
pubmed:abstractText
In a previous study we explored the inducibility of erythroid, granulocytic, monocytic and megakaryocytic antigens in the human multipotent leukemia cell line K562. In this study we examine the relationship between induction into the two lineages for which inducibility is most marked: erythroid and megakaryocytic types. Specifically, does any cell manifest markers of both lineages? We show that 1) cultured without inducer, a minority of single cells expresses both erythroid and megakaryocytic antigens, i.e., glycophorin A and Plt-1 antigen, 2) thymidine-hypoxanthine and phorbol dibutyrate each induce each antigen, 3) the percentage of individual cells carrying both antigens increases under each of these inducing conditions, 4) the induction of each is not only relative in terms of percentage of total cells that are positive, but also absolute in terms of mean antigen per cell and (taking into account cell multiplication) total antigen per ml of culture, and 5) the inducibility of individual cells bearing both erythroid and megakaryocytic markers is confirmed by using a different inducer (butyric acid) and antibodies for different inducer (butyric acid) and antibodies for different erythroid and megakaryocytic antigens (VIE-G4 and AP-3, respectively).
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0737-1454
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
232-40
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Single K562 human leukemia cells express and are inducible for both erythroid and megakaryocytic antigens.
pubmed:affiliation
Department of Medicine, University of Rochester, School of Medicine, NY 14642.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't