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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2006-5-15
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pubmed:abstractText |
Fesselin is an actin binding protein from smooth muscle that nucleates actin polymerization in a Ca(++)-calmodulin dependent manner, bundles actin and inhibits the actin-activated ATPase activity of myosin S1. We now report that fesselin binds to smooth muscle alpha-actinin. Binding was measured by blot overlay, affinity chromatography and sedimentation methods. Binding was moderate with an association constant of 1-4 x 10(7) M(-1) assuming a 1:1 association of fesselin with alpha-actinin. Fesselin binds to the central spectrin domain repeat region of alpha-actinin but not to the CH1-CH2 domain. Fesselin accelerates the polymerization of actin. This activity of fesselin was attenuated by alpha-actinin. These observations support the role of fesselin in organizing the cytoskeleton.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0142-4319
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
27
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
45-51
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:16450054-Actin Cytoskeleton,
pubmed-meshheading:16450054-Actinin,
pubmed-meshheading:16450054-Animals,
pubmed-meshheading:16450054-Binding Sites,
pubmed-meshheading:16450054-Biopolymers,
pubmed-meshheading:16450054-Cattle,
pubmed-meshheading:16450054-Cytoskeleton,
pubmed-meshheading:16450054-Membrane Proteins,
pubmed-meshheading:16450054-Microfilament Proteins,
pubmed-meshheading:16450054-Muscle, Smooth,
pubmed-meshheading:16450054-Protein Binding,
pubmed-meshheading:16450054-Protein Structure, Tertiary,
pubmed-meshheading:16450054-Rabbits,
pubmed-meshheading:16450054-Turkeys
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pubmed:year |
2006
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pubmed:articleTitle |
Smooth muscle alpha-actinin binds tightly to fesselin and attenuates its activity toward actin polymerization.
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pubmed:affiliation |
Brody School of Medicine at East Carolina University, 600 Moye Blvd., Greenville, NC 27834, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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